Laboratoire des Bioprocédés Environnementaux, Pôle d'Excellence Régional (PER, AUF), Centre de Biotechnologie de Sfax, Université de Sfax, B.P. 1117, 3018 Sfax, Tunisia.
Bioresour Technol. 2011 Oct;102(19):9050-8. doi: 10.1016/j.biortech.2011.07.048. Epub 2011 Jul 24.
Aspergillus niger broth culture on wheat bran was assessed for olive wastewater (OW) hydrolysis in order to release hydroxytyrosol (HT). The enzyme profiles of this culture broth gave essentially (IU/L): 3000 β-glucosidase and 100 esterase. Hydrolysis activity of A. niger enzyme preparation was evaluated by using three substrates: raw OW, phenolic fraction extracted from OW by ethyl acetate and its corresponding exhausted fraction. Large amounts of free simple phenolics were released from exhausted fraction and raw OW after enzymatic treatment. HPLC analyses show that HT was the main phenolic compound. One step of ethyl acetate extraction of hydrolysed OW allowed the recovery of 0.8 g of HT per litre of OW. The antioxidant activity of extracts from OW and exhausted fraction, measured by DPPH method, was drastically enhanced after hydrolysis treatment. This study demonstrates that hydrolysed OW is a potential source of bioactive phenolic compounds with promising applications in food and pharmaceutical industries.
黑曲霉在麦麸上的液体培养物被评估用于橄榄废水 (OW) 的水解，以释放羟基酪醇 (HT)。该培养物的酶谱主要为（IU/L）：3000 个β-葡萄糖苷酶和 100 个酯酶。通过使用三种底物：原 OW、用乙酸乙酯从 OW 中提取的酚类部分及其相应的耗尽部分，评估了黑曲霉酶制剂的水解活性。酶处理后，大量的游离简单酚类物质从耗尽部分和原 OW 中释放出来。HPLC 分析表明 HT 是主要的酚类化合物。一步法用乙酸乙酯从水解的 OW 中提取，每升 OW 可回收 0.8 克 HT。用 DPPH 法测定 OW 和耗尽部分提取物的抗氧化活性，水解处理后明显增强。这项研究表明，水解的 OW 是生物活性酚类化合物的潜在来源，在食品和制药工业中有很好的应用前景。
