Department of Molecular Microbiology, Institute of Experimental Medicine, St. Petersburg, Russia.
Benef Microbes. 2011 Mar;2(1):9-13. doi: 10.3920/BM2010.0006.
Antibacterial activity of probiotic enterococci is often related to the production of bacteriocins. There is a variety of Enterococcus faecium strains which synthesise one or several enterocins including A, B, P, 96, L50AB and others. In this work we have analysed the probiotic strain E. faecium L3 whose genome contained the genes encoding enterocins А, В and a three-component regulatory system. All these genes were found to be expressed in enterococcal strain L3. Antimicrobial activity of the strain tested on the strains of Listeria monocytogenes and Streptococcus agalactiae was pheromone dependent. Chemically synthesised cyclic peptides (analogues and shorter derivates of EntF) with different molecular structures were able to increase the antagonistic activity of the strain E. faecium L3 in contrast to the strain E. faecium SF68, which does nоt have genes encoding enterocins А and В.
益生菌肠球菌的抗菌活性通常与细菌素的产生有关。有多种屎肠球菌菌株可以合成一种或多种肠球菌素，包括 A、B、P、96、L50AB 等。在这项工作中，我们分析了益生菌菌株 E. faecium L3，其基因组包含编码肠球菌素 A、B 和三组分调节系统的基因。所有这些基因都在肠球菌株 L3 中表达。在李斯特菌和无乳链球菌菌株上测试的菌株的抗菌活性依赖于信息素。用不同分子结构合成的化学合成环肽（EntF 的类似物和较短的衍生物）能够增加菌株 E. faecium L3 的拮抗活性，而不具有编码肠球菌素 A 和 B 的基因的菌株 E. faecium SF68 则不能。
