Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Pr. 100 Let Vladivostoku 159, 690022 Vladivostok, Russia.
Far Eastern Federal University, Sukhanova St. 8, 690950 Vladivostok, Russia.
Int J Syst Evol Microbiol. 2012 Jul;62(Pt 7):1450-1456. doi: 10.1099/ijs.0.032219-0. Epub 2011 Aug 12.
A Gram-negative, facultatively anaerobic, slightly halophilic bacterium that was motile by gliding, designated KMM 6390(T), was isolated from the coastal green alga Ulva fenestrata collected from the Sea of Japan. A comparative analysis of 16S rRNA gene sequences revealed that strain KMM 6390(T) was a member of the genus Winogradskyella, with Winogradskyella echinorum KMM 6211(T) as its closest relative (97.8% 16S rRNA gene sequence similarity). 16S rRNA gene sequence similarities between the isolate and other members of the genus Winogradskyella were 95.4-96.7%. DNA-DNA relatedness between the isolate and W. echinorum KMM 6211(T) was 27%. Strain KMM 6390(T) was able to grow with 0.5-4.0% NaCl and at 4-37 °C. The isolate decomposed casein, gelatin, starch and DNA and produced acid from galactose, D-glucose, lactose and maltose. The major fatty acids were iso-C(15:0), iso-C(15:1), anteiso-C(15:0) and C(15:0). The polar lipid profile comprised phosphatidylethanolamine and two unknown aminolipids. The DNA G+C content was 34.2 mol%. On the basis of phylogenetic, chemotaxonomic and phenotypic investigations, strain KMM 6390(T) represents a novel species of the genus Winogradskyella, for which the name Winogradskyella ulvae sp. nov. is proposed; the type strain is KMM 6390(T) (=KCTC 23626(T)=LMG 26444(T)). Emended descriptions of the genus Winogradskyella and of W. thalassocola, W. echinorum, W. exilis and W. eximia are also provided.
一株革兰氏阴性、兼性厌氧、轻度嗜盐的滑行运动细菌，被命名为 KMM 6390(T)，从日本海采集的沿海绿藻石莼中分离得到。16S rRNA 基因序列的比较分析表明，菌株 KMM 6390(T)是 Winogradskyella 属的一员，与 W. echinorum KMM 6211(T)最为接近（16S rRNA 基因序列相似度为 97.8%）。该分离株与其他 Winogradskyella 属成员的 16S rRNA 基因序列相似度为 95.4-96.7%。该分离株与 W. echinorum KMM 6211(T)的 DNA-DNA 相关性为 27%。KMM 6390(T)菌株能够在 0.5-4.0%NaCl 和 4-37°C 的条件下生长。该分离株能够分解酪蛋白、明胶、淀粉和 DNA，并从半乳糖、D-葡萄糖、乳糖和麦芽糖中产生酸。主要脂肪酸为 iso-C(15:0)、iso-C(15:1)、anteiso-C(15:0)和 C(15:0)。极性脂谱由磷脂酰乙醇胺和两种未知的氨基脂组成。DNA G+C 含量为 34.2mol%。基于系统发育、化学分类和表型研究，KMM 6390(T)菌株代表了 Winogradskyella 属的一个新种，建议将其命名为 Winogradskyella ulvae sp. nov.；其模式菌株为 KMM 6390(T)（=KCTC 23626(T)=LMG 26444(T)）。还对 Winogradskyella 属和 W. thalassocola、W. echinorum、W. exilis 和 W. eximia 的描述进行了修订。
