Nedashkovskaya Olga I, Kukhlevskiy Andrey D, Zhukova Natalia V, Kim So-Jeong, Rhee Sung-Keun, Mikhailov Valery V
Far Eastern Federal University, Sukhanova St. 8, 690950, Vladivostok, Russia.
G.B. Elyakov Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Pr. 100 Let Vladivostoku 159, 690022, Vladivostok, Russia.
Int J Syst Evol Microbiol. 2015 Oct;65(10):3652-3657. doi: 10.1099/ijsem.0.000470.
A Gram-stain-negative, facultatively anaerobic, moderately halophilic and gliding bacterium, designated KMM 6491T, was isolated from coastal seawater collected from Troitsa Bay, the Sea of Japan. Comparative analysis of 16S rRNA gene sequences revealed that strain KMM 6491T was a member of the genus Winogradskyella, with 94.5–97.9 % sequence similarity to recognized species of the genus Winogradskyella. The DNA G+C content of strain KMM 6491T was 31.3 mol% and DNA–DNA relatedness values with the type strains of Winogradskyella echinorum, Winogradskyella damuponensis, Winogradskyella eximia and Winogradskyella pulchriflava were in range of 10–26 %. Strain KMM 6491T contained menaquinone 6 (MK-6) as the single quinone and iso-C15 : 0, iso-C15 : 1, iso-C15 : 0 3-OH, iso-C17 : 0 3-OH, C15 : 0 and summed feature 3 (comprising iso-C15 : 0 2-OH and/or C16 : 1ω7c) anteiso-C15 : 0, as the prevalent fatty acids. The major polar lipids of strain KMM 6491T were phosphatidylethanolamine, two unknown aminolipids and two unknown lipids. Strain KMM 6491T was able to grow with 0.5–7 % NaCl and at 4–34 °C. The novel strain decomposed gelatin and starch and produced acid from d-glucose, maltose, mannose, rhamnose, sucrose, fructose and glycerol. On the basis of the results of the phylogenetic and phenotypic analyses it is suggested that strain KMM 6491T represents a novel species of the genus Winogradskyella, for which the name Winogradskyella litoriviva sp. nov. is proposed. The type strain is KMM 6491T ( = KCTC 23972T = LMG 26984T).
从日本海特罗伊察湾采集的沿海海水中分离出一株革兰氏染色阴性、兼性厌氧、中度嗜盐且能滑行的细菌，命名为KMM 6491T。16S rRNA基因序列的比较分析表明，菌株KMM 6491T是维诺格拉德斯基氏菌属的成员，与维诺格拉德斯基氏菌属已确认物种的序列相似性为94.5 - 97.9%。菌株KMM 6491T的DNA G + C含量为31.3 mol%，与棘刺维诺格拉德斯基氏菌、达蒙维诺格拉德斯基氏菌、优美维诺格拉德斯基氏菌和美丽维诺格拉德斯基氏菌模式菌株的DNA - DNA相关性值在10 - 26%范围内。菌株KMM 6491T含有甲基萘醌6（MK - 6）作为唯一醌类，以及异C15 : 0、异C15 : 1、异C15 : 0 3 - OH、异C17 : 0 3 - OH、C15 : 0和总和特征3（包括异C15 : 0 2 - OH和/或C16 : 1ω7c）、前异C15 : 0作为主要脂肪酸。菌株KMM 6491T的主要极性脂类为磷脂酰乙醇胺、两种未知氨基脂类和两种未知脂类。菌株KMM 6491T能够在0.5 - 7% NaCl浓度以及4 - 34°C条件下生长。该新菌株能分解明胶和淀粉，可从d - 葡萄糖、麦芽糖、甘露糖、鼠李糖、蔗糖、果糖和甘油产酸。基于系统发育和表型分析结果，建议菌株KMM 6491T代表维诺格拉德斯基氏菌属的一个新物种，为此提出新名称滨海维诺格拉德斯基氏菌（Winogradskyella litoriviva sp. nov.）。模式菌株为KMM 6491T（= KCTC 23972T = LMG 26984T）。
