Department of Veterinary Disease Biology, Faculty of Life Sciences, University of Copenhagen, DK-1870 Frederiksberg C, Denmark.
Center for Zoo and Wild Animal Health, Copenhagen Zoo, DK-2000 Frederiksberg, Denmark.
Int J Syst Evol Microbiol. 2012 Jun;62(Pt 6):1396-1401. doi: 10.1099/ijs.0.035246-0. Epub 2011 Aug 12.
Pasteurella species B has so far only been reported from the oral cavity of dogs, cats and a ferret. In the present study, information from 15 recent isolates from different sources, including African hedgehogs (Atelerix albiventris), banded mongoose (Mungos mungo), Moholi bushbabies (Galago moholi) and pneumonia of a cat, were compared to five strains investigated previously from bite wounds in humans inflicted by a cat and dog and from gingiva of a cat. rpoB gene sequence comparison showed that 17 isolates, including the reference strain (CCUG 19794(T)), had identical sequences, whereas two were closely related and demonstrated 97.9 and 99.6 % similarity to strain CCUG 19794(T), respectively; the type strain of Pasteurella stomatis was the most closely related strain, with 92.3 % similarity. This is within the mean range (76-100 %) of rpoB gene sequence similarity between species of the same genus within the family Pasteurellaceae. 16S rRNA gene sequencing of four strains selected based on rpoB sequence comparison showed at least 99.7 % similarity between strains of Pasteurella species B, with 96.2 % similarity to the type strain of the closest related species (Pasteurella canis), indicating that Pasteurella species B should have separate species status. Separate species status was also documented when recN sequence comparisons were converted to a genome similarity of 93.7 % within Pasteurella species B and 59.0 % to the type strain of the closest related species (P. canis). Based on analysis of the phylogenetic and phenotypic data, and since most isolates originate from the oral cavities of a diverse group of animals, it is suggested that these bacteria be classified as Pasteurella oralis sp. nov.; the type strain is P683(T) ( = CCUG 19794(T) = CCM 7950(T) = strain 23193(T) = MCCM 00102(T)), obtained from a cat. Previous reports of the type strain have shown ubiquinone-8, demethylmenaquinone-8 and menaquinone-8 as the major quinones. Polyamines in the type strain were reported as diaminopropane, putrescine, cadaverine, sym-norspermidine, spermidine and spermine in a previous investigation, and the major fatty acids of the type strain were reported to be C(16:0), C(16:1)ω7c and C(14:0), with minor amounts of C(18:0) and C(18:1)ω9c. The DNA G+C content of the type strain has been reported to be 40.0 mol%.
巴斯德氏菌 B 种迄今仅报道于狗、猫和雪貂的口腔中。在本研究中，对来自不同来源的 15 株近期分离株（包括非洲刺猬（Atelerix albiventris）、条纹獴（Mungos mungo）、莫霍利 Bushbaby（Galago moholi）和猫肺炎）的信息，与之前从猫咬伤人和猫牙龈炎中分离的 5 株进行了比较。rpoB 基因序列比较显示，包括参考株（CCUG 19794(T)）在内的 17 株分离株具有相同的序列，而另外 2 株则密切相关，与 CCUG 19794(T)的相似度分别为 97.9%和 99.6%；牙龈巴斯德氏菌（Pasteurella stomatis）的模式株是最密切相关的菌株，相似度为 92.3%。这在同一科巴斯德氏菌属内相同属种的 rpoB 基因序列相似性均值范围内（76-100%）。基于 rpoB 序列比较选择的 4 株菌的 16S rRNA 基因测序显示，巴斯德氏菌 B 种之间的菌株相似度至少为 99.7%，与最密切相关种（犬巴斯德氏菌（Pasteurella canis））的模式株相似度为 96.2%，表明巴斯德氏菌 B 种应具有独立的种地位。当将 recN 序列比较转换为巴斯德氏菌 B 种内 93.7%的基因组相似性和与最密切相关种（犬巴斯德氏菌（P. canis））的模式株 59.0%的基因组相似性时，也记录了独立的种地位。基于系统发育和表型数据分析，由于大多数分离株源自一组不同动物的口腔，因此建议将这些细菌分类为口腔巴斯德氏菌（Pasteurella oralis）sp. nov.；模式株为 P683(T)（=CCUG 19794(T)=CCM 7950(T)=菌株 23193(T)=MCCM 00102(T)），从猫中分离得到。先前对模式株的报道表明，泛醌-8、脱甲基menaquinone-8 和menaquinone-8 为主要的类异戊二烯醌。先前的研究报道，模式株中的多胺为二氨基丙胺、腐胺、尸胺、sym-norspermidine、亚精胺和精胺，模式株中的主要脂肪酸为 C(16:0)、C(16:1)ω7c 和 C(14:0)，少量 C(18:0)和 C(18:1)ω9c。先前的报道称，模式株的 DNA G+C 含量为 40.0 mol%。
