Martín-Quintal Zhelmy, Moo-Puc Rosa, González-Salazar Francisco, Chan-Bacab Manuel J, Torres-Tapia Luis W, Peraza-Sánchez Sergio R
Centro de Investigación Científica de Yucatán, Mérida, Yucatán(CICY), Mexico.
J Ethnopharmacol. 2009 Apr 21;122(3):463-7. doi: 10.1016/j.jep.2009.01.037. Epub 2009 Feb 10.
Tridax procumbens is an active herb against leishmaniasis.
Leishmaniasis is a group of diseases caused by Leishmania protozoa. We investigated the antileishmanial activity of Tridax procumbens extracts and a pure compound against promastigotes of Leishmania mexicana, the causative agent of cutaneous leishmaniasis in the New World.
Extracts and (3S)-16,17-didehydrofalcarinol (1) were obtained by chromatographic methods from Tridax procumbens, and the latter identified by spectroscopic analysis. The effect of these extracts and 1 on the growth inhibition of promastigotes of Leishmania mexicana was evaluated. In order to test the safety of extracts and 1, mammalian cells were treated with them, and cell viability was assessed using trypan blue and MTT.
We demonstrated that extracts of Tridax procumbens and 1 showed a pronounced activity against Leishmania mexicana. The methanol extract inhibited promastigotes growth of Leishmania mexicana with a 50% inhibitory concentration (IC(50)) of 3 microg/ml, while oxylipin 1 exhibited the highest inhibition at IC(50)=0.478 microg/ml.
In this study we report the biological activity of extracts and (3S)-16,17-didehydrofalcarinol (1), obtained from Tridax procumbens, on the promastigote form of Leishmania mexicana, with no effect upon mammalian cells.
平卧银胶菊是一种对利什曼病有效的草药。
利什曼病是由利什曼原虫引起的一组疾病。我们研究了平卧银胶菊提取物和一种纯化合物对墨西哥利什曼原虫前鞭毛体的抗利什曼活性，墨西哥利什曼原虫是新世界皮肤利什曼病的病原体。
通过色谱法从平卧银胶菊中获得提取物和(3S)-16,17-二脱氢法卡林醇(1)，后者通过光谱分析进行鉴定。评估了这些提取物和1对墨西哥利什曼原虫前鞭毛体生长抑制的作用。为了测试提取物和1的安全性，用它们处理哺乳动物细胞，并使用台盼蓝和MTT评估细胞活力。
我们证明平卧银胶菊提取物和1对墨西哥利什曼原虫显示出显著活性。甲醇提取物抑制墨西哥利什曼原虫前鞭毛体生长，其50%抑制浓度(IC(50))为3微克/毫升，而氧化脂质1在IC(50)=0.478微克/毫升时表现出最高抑制作用。
在本研究中，我们报道了从平卧银胶菊中获得的提取物和(3S)-16,17-二脱氢法卡林醇(1)对墨西哥利什曼原虫前鞭毛体形式的生物活性，对哺乳动物细胞无影响。
