利用光子反聚束测定水合状态下重组高密度脂蛋白的化学计量比。
Stoichiometry of reconstituted high-density lipoproteins in the hydrated state determined by photon antibunching.
机构信息
Physical and Life Science Directorate, Lawrence Livermore National Laboratory, Livermore, California, USA.
出版信息
Biophys J. 2011 Aug 17;101(4):970-5. doi: 10.1016/j.bpj.2011.06.060.
Abstract
Apolipoprotein A-I plays a central role in the solution structure of high-density lipoproteins. Determining the stoichiometry of lipid-bound apo A-I in the hydrated state is therefore fundamental to understanding how high-density lipoproteins form and function. Here, we use the quantum optical phenomenon of photon antibunching to determine the number of apo A-I molecules bound to discoidal lipoproteins and compare this with values obtained by photon-counting histogram analysis. Both the photon antibunching and photon-counting analyses show that reconstituted high-density lipoprotein particles contain two apo A-I molecules, which is in agreement with the commonly accepted double-belt model.
摘要
载脂蛋白 A-I 在高密度脂蛋白的溶液结构中起着核心作用。因此，确定水合状态下与脂质结合的载脂蛋白 A-I 的化学计量对于理解高密度脂蛋白的形成和功能至关重要。在这里，我们利用光子反聚束的量子光学现象来确定结合在盘状脂蛋白上的载脂蛋白 A-I 分子的数量，并将其与通过光子计数直方图分析获得的值进行比较。光子反聚束和光子计数分析都表明，重组的高密度脂蛋白颗粒含有两个载脂蛋白 A-I 分子，这与普遍接受的双带模型一致。
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