Department of Developmental and Regenerative Biology, Mount Sinai School of Medicine, New York, NY 10029, USA.
Mech Dev. 2011 Sep-Dec;128(7-10):376-86. doi: 10.1016/j.mod.2011.07.004. Epub 2011 Aug 6.
The centrosome is essential for the formation of the cilia and has been implicated in cell polarization and signaling during early embryonic development. A number of Wnt pathway components were found to localize at the centrosome, but how this localization relates to their signaling functions is unclear. In this study, we assessed a role for Diversin, a putative Wnt pathway mediator, in developmental processes that involve cilia. We find that Diversin is specifically localized to the basal body compartment near the base of the cilium in Xenopus multi-ciliated skin cells. Overexpression of Diversin RNA disrupted basal body polarization in these cells, suggesting that tightly regulated control of Diversin levels is crucial for this process. In cells depleted of endogenous Diversin, basal body structure appeared abnormal and this was accompanied by disrupted polarity, shortened or absent cilia and defective ciliary flow. These results are consistent with the involvement of Diversin in processes that are related to the acquisition of cell polarity and require ciliary functions.
中心体对于纤毛的形成是必不可少的，并且在早期胚胎发育过程中的细胞极化和信号转导中也发挥作用。许多 Wnt 信号通路的成分被发现定位于中心体，但这种定位与其信号转导功能的关系尚不清楚。在这项研究中，我们评估了 Diversin 在涉及纤毛的发育过程中的作用，Diversin 是一种假定的 Wnt 信号通路介质。我们发现，Diversin 特异性地定位于 Xenopus 多纤毛皮肤细胞的纤毛底部附近的基底体隔室。Diversin RNA 的过表达扰乱了这些细胞中的基底体极化，这表明对 Diversin 水平的严格调控对于该过程至关重要。在耗尽内源性 Diversin 的细胞中，基底体结构似乎异常，这伴随着极性紊乱、纤毛缩短或缺失以及纤毛流缺陷。这些结果与 Diversin 参与与细胞极性获得相关的过程以及需要纤毛功能的过程一致。
