Kilic Abdullah, Aktas Zerin, Bedir Orhan, Gumral Ramazan, Bulut Yasemin, Stratton Charles, Tang Yi-Wei, Basustaoglu A Celal
Departments of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy and School of Medicine, Ankara, Turkey.
Ann Clin Lab Sci. 2011 Spring;41(2):161-6.
Carbapenem resistance in Enterobacteriaceae isolates has been reported from Turkey and is most often mediated by OXA-48 type carbapenemases. We report the identification and characterization of four carbapenem-resistant isolates (three Klebsiella pneumoniae and one Escherichia coli) among 515 clinical Enterobacteriaceae isolates collected during a 7-month study period in Ankara, Turkey. The four isolates were recovered from blood and urine specimens in patients with varied clinical manifestations. They had distinct pulsed-field gel electrophoresis patterns and harbored a variety of β-lactamases including bla(TEM-1), bla(SHV-12) genes, bla(SHV-11), and/or bla(CTX-M-15). PCR and sequencing analysis revealed that the bla(OXA-48) gene was present in all four isolates. Our data indicated that the OXA-48-type carbapenemase was the only mechanism for carbapenem resistance in our hospital.
土耳其已报道肠杆菌科分离株对碳青霉烯类耐药，且大多由OXA - 48型碳青霉烯酶介导。我们报告了在土耳其安卡拉进行的为期7个月的研究期间收集的515株临床肠杆菌科分离株中，鉴定并表征了4株耐碳青霉烯分离株（3株肺炎克雷伯菌和1株大肠埃希菌）。这4株分离株是从临床表现各异的患者的血液和尿液标本中分离出来的。它们具有不同的脉冲场凝胶电泳图谱，并携带多种β - 内酰胺酶，包括bla(TEM - 1)、bla(SHV - 12)基因、bla(SHV - 11)和/或bla(CTX - M - 15)。PCR和测序分析表明，所有4株分离株均存在bla(OXA - 48)基因。我们的数据表明，OXA - 48型碳青霉烯酶是我院碳青霉烯耐药的唯一机制。
