Ali Nusrat, Datta Swapan K, Datta Karabi
Plant Molecular Biology and Biotechnology Laboratory, Department of Botany, University of Calcutta, India.
GM Crops. 2010 Jul-Sep;1(4):207-13. doi: 10.4161/gmcr.1.4.13344.
RNA interference (RNAi) is a sequence specific gene silencing mechanism, triggered by the introduction of dsRNA leading to mRNA degradation. It helps in switching on and off the targeted gene, which might have significant impact in developmental biology. Discovery of RNAi represents one of the most promising and rapidly advancing frontiers in plant functional genomics and in crop improvement by plant metabolic engineering and also plays an important role in reduction of allergenicity by silencing specific plant allergens. In plants the RNAi technology has been employed successfully in improvement of several plant species- by increasing their nutritional value, overall quality and by conferring resistance against pathogens and diseases. The review gives an insight to the perspective use of the technology in designing crops with innovation, to bring improvement to crop productivity and quality.
RNA干扰（RNAi）是一种序列特异性基因沉默机制，由双链RNA的引入引发，导致mRNA降解。它有助于开启和关闭靶向基因，这可能对发育生物学产生重大影响。RNAi的发现是植物功能基因组学以及通过植物代谢工程进行作物改良中最有前景且发展迅速的前沿领域之一，并且在通过沉默特定植物过敏原降低致敏性方面也发挥着重要作用。在植物中，RNAi技术已成功应用于多种植物物种的改良——提高其营养价值、整体品质，并赋予对病原体和疾病的抗性。这篇综述深入探讨了该技术在创新设计作物以提高作物生产力和品质方面的潜在用途。
