College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, 150030, Harbin, China.
Avian Pathol. 2011 Oct;40(5):491-8. doi: 10.1080/03079457.2011.605107. Epub 2011 Aug 18.
Avian infectious bronchitis virus (IBV), a coronavirus, causes infectious bronchitis leading to enormous economic loss in the poultry industry worldwide. Houttuynia cordata (Saururaceae) (HC) is a traditional Chinese medicine used in China. In the present study, the effect of HC on cell infection by IBV was determined using plaque assay and reverse transcription-polymerase chain reaction. The inhibitory effect of HC on IBV infection in ovo and in vivo was analysed using specific pathogen free (SPF) chicken embryos and chickens. Moreover, the effect of HC on cell apoptosis induced by IBV was investigated. Results showed that HC had more than 90% inhibition rate against IBV infection in Vero cells and chicken embryo kidney cells, and decreased more than 90% apoptotic cells caused by IBV. HC fully protected the SPF embryos, and had more than 50% protection rate in SPF chickens, against IBV challenge.
禽传染性支气管炎病毒（IBV）是冠状病毒的一种，可引起传染性支气管炎，给全球家禽业造成巨大经济损失。鱼腥草（三白草科）（HC）是中国传统中药。本研究采用噬斑法和逆转录-聚合酶链反应（RT-PCR），检测 HC 对 IBV 感染细胞的作用。采用无特定病原体（SPF）鸡胚和鸡分析 HC 对鸡胚和鸡体内 IBV 感染的抑制作用。此外，还研究了 HC 对 IBV 诱导的细胞凋亡的影响。结果表明，HC 对 IBV 感染 Vero 细胞和鸡胚肾细胞的抑制率超过 90%，并降低了超过 90%的 IBV 诱导的凋亡细胞。HC 能完全保护 SPF 鸡胚，对 SPF 鸡的保护率超过 50%，能抵抗 IBV 攻击。
