Scottish Haemophilus Legionella Meningococcus Pneumococcus Reference Laboratory (SHLMPRL), House on the Hill, Stobhill Hospital, Glasgow, UK.
Clin Microbiol Infect. 2011 Oct;17(10):1517-20. doi: 10.1111/j.1469-0691.2011.03582.x. Epub 2011 Aug 16.
Legionella pneumophila and Legionella species were isolated from composted material when freshly prepared buffered charcoal yeast extract (BCYE) was supplemented with glycine (1.5 g/L), polymyxin B sulfate (40 000 IU/L), vancomycin hydrochloride (0.5 mg/L) and cycloheximide (40 mg/L) (GVPC medium) and Modified Wadowsky-Yee (MWY) (Oxoid, Cambridge, UK) plates were used for cultivation, but not with commercially sourced pre-poured GVPC and MWY plates (Oxoid). Legionella cincinnatiensis and pathogenic L. pneumophila serogroup (Sg) 1 Benidorm and France/Allentown were identified, as well as a non-typeable (NT) strain of L. pneumophila. As most laboratories no longer produce their own media, this may contribute to the lack of positive cultures from composted material. The antigenicity of the NT strain is discussed.
当新鲜制备的缓冲炭酵母提取物 (BCYE) 中添加甘氨酸 (1.5 g/L)、硫酸多黏菌素 B (40000 IU/L)、盐酸万古霉素 (0.5 mg/L) 和环己酰亚胺 (40 mg/L) (GVPC 培养基) 并且使用改良 Wadowsky-Yee (MWY) (Oxoid，英国剑桥) 平板进行培养时，从堆肥材料中分离出嗜肺军团菌和军团菌属物种，但使用市售的预制 GVPC 和 MWY 平板 (Oxoid) 则无法分离。鉴定出辛辛那提军团菌和致病性嗜肺军团菌血清群 (Sg) 1 贝尼多姆和法国/阿伦镇，以及一株不可分型 (NT) 的嗜肺军团菌。由于大多数实验室不再自己制备培养基，这可能导致从堆肥材料中培养不出阳性结果。还讨论了 NT 菌株的抗原性。
