Wadowsky R M, Yee R B
Appl Environ Microbiol. 1981 Nov;42(5):768-72. doi: 10.1128/aem.42.5.768-772.1981.
Glycine, at a final concentration of 0.3%, has been shown to be an excellent selective agent for the isolation of Legionellaceae. Stock cultures of Legionella pneumophila were not inhibited on buffered charcoal-yeast extract agar containing the amino acid. Among the other Legionellaceae tested, only one of two strains of L. dumoffii and two of six strains of L. micdadei were appreciably inhibited. This medium permitted the isolation of L. pneumophila from environmental specimens with marked inhibition of many non-Legionellaceae bacteria. The selectivity of the medium was subsequently improved by the incorporation of vancomycin (5 microgram/ml) and polymyxin B (100 U/ml). This selective medium, glycine-vancomycin-polymyxin B agar, should facilitate the recovery of Legionellaceae from environmental sources.
已证明，终浓度为0.3%的甘氨酸是用于分离军团菌科的优良选择剂。嗜肺军团菌的储备培养物在含有该氨基酸的缓冲活性炭酵母提取物琼脂上未受到抑制。在所测试的其他军团菌科中，杜莫夫军团菌的两株菌株中只有一株以及米克戴德军团菌的六株菌株中有两株受到明显抑制。这种培养基可从环境标本中分离出嗜肺军团菌，同时对许多非军团菌科细菌有明显抑制作用。随后通过加入万古霉素（5微克/毫升）和多粘菌素B（100单位/毫升）提高了培养基的选择性。这种选择性培养基，即甘氨酸-万古霉素-多粘菌素B琼脂，应有助于从环境来源中分离出军团菌科。
