Centre for Immunity, Infection and Evolution, University of Edinburgh, Kings Buildings, West Mains Road, Edinburgh EH9 3JT, UK.
J R Soc Interface. 2012 Mar 7;9(68):518-27. doi: 10.1098/rsif.2011.0293. Epub 2011 Aug 17.
Mathematical model-based statistical inference applied to within-host dynamics of infectious diseases can help dissect complex interactions between hosts and microbes. This work has applied advances in model-based inference to understand colonization of cattle by enterohaemorrhagic Escherichia coli O157 : H7 at the terminal rectum. A mathematical model was developed based on niche replication and transition rates at this site. A nested-model comparison, applied to excretion curves from 25 calves, was used to reduce complexity while maintaining integrity. We conclude that, 5-9 days post inoculation, the innate immune response negates bacterial replication on the epithelium and either reduces attachment to or increases detachment from the epithelium of the terminal rectum. Thus, we provide a broadly applicable model that gives novel insights into bacterial replication rates in vivo and the timing and impact of host responses.
基于数学模型的统计推断应用于传染病的宿主内动态，可以帮助剖析宿主和微生物之间的复杂相互作用。这项工作应用了基于模型的推断方法的进展，以了解肠出血性大肠杆菌 O157：H7 在终直肠的牛群定植情况。基于该部位的生态位复制和转换率，建立了一个数学模型。通过嵌套模型比较，应用于 25 头小牛的排泄曲线，在保持完整性的同时减少了复杂性。我们得出结论，在接种后 5-9 天，先天免疫反应否定了上皮细胞的细菌复制，要么减少了对终直肠上皮的附着，要么增加了对其的脱落。因此，我们提供了一个广泛适用的模型，为体内细菌复制率以及宿主反应的时间和影响提供了新的见解。
