HSPA1A的变体与血浆热休克蛋白70（Hsp70）及抗Hsp70抗体水平相结合，与急性冠状动脉综合征的较高风险相关。

Variants of HSPA1A in combination with plasma Hsp70 and anti-Hsp70 antibody levels associated with higher risk of acute coronary syndrome.

作者信息

Zhang Xiaomin, Tanguay Robert M, He Mei'an, Deng Qifei, Miao Xiaoping, Zhou Li, Wu Tangchun

机构信息

Department of Occupational and Environmental Health and the Ministry of Education Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

出版信息

Cardiology. 2011;119(1):57-64. doi: 10.1159/000329917. Epub 2011 Aug 17.

DOI:10.1159/000329917

Abstract

OBJECTIVES

It was the aim of our study to investigate whether polymorphisms of HSP70 have an affect on antigen and antibody levels in acute coronary syndrome (ACS) patients and normal controls, and the possible joint effect of variants and antigen and antibody levels on the risk of ACS.

METHODS

Three single nucleotide polymorphisms of HSPA1A and HSPA1L were evaluated in 520 ACS patients and 520 age- and sex-matched controls. Plasma extracellular Hsp70 (eHsp70) and anti-Hsp70 antibody levels were determined using ELISA.

RESULTS

Individuals with +190G/C (rs1043618) CC genotype in HSPA1A had higher levels of eHsp70 in controls and lower levels of anti-Hsp70 body in ACS, compared with +190G/C GG carriers. Significantly increased ACS risks of 2.93 and 3.53 fold were found in subjects with the +190G/C CC genotype and high eHsp70 levels or low anti-Hsp70 antibody levels, respectively. The highest risk of ACS was found in subjects with +190G/C CC genotypes, high eHsp70 and low anti-Hsp70 antibody levels compared with those in the reference group (OR = 7.57, 95% CI 3.04-18.87).

CONCLUSIONS

The +190G/C polymorphism of HSPA1A may contribute to influence eHsp70 levels in controls and anti-Hsp70 antibody levels in ACS, and the +190G/C genotypes, eHsp70 and anti-Hsp70 antibody levels may have a joint effect on the risk of ACS.

摘要

目的

本研究旨在调查热休克蛋白70（HSP70）基因多态性是否影响急性冠状动脉综合征（ACS）患者及正常对照者的抗原和抗体水平，以及基因变异与抗原和抗体水平对ACS风险的可能联合作用。

方法

对520例ACS患者和520例年龄及性别匹配的对照者评估热休克蛋白A1A（HSPA1A）和热休克蛋白A1L（HSPA1L）的三个单核苷酸多态性。采用酶联免疫吸附测定法（ELISA）测定血浆细胞外Hsp70（eHsp70）和抗Hsp70抗体水平。

结果

与+190G/C GG基因型携带者相比，HSPA1A基因+190G/C（rs1043618）CC基因型的对照者eHsp70水平较高，而ACS患者抗Hsp70抗体水平较低。+190G/C CC基因型且eHsp70水平高或抗Hsp70抗体水平低的受试者发生ACS的风险分别显著增加2.93倍和3.53倍。与参照组相比，+190G/C CC基因型、eHsp70水平高且抗Hsp70抗体水平低的受试者发生ACS的风险最高（比值比=7.57，95%可信区间3.04 - 18.87）。

结论

HSPA1A基因的+190G/C多态性可能影响对照者的eHsp70水平和ACS患者的抗Hsp70抗体水平，+190G/C基因型、eHsp70和抗Hsp70抗体水平可能对ACS风险产生联合作用。