Department of Natural Sciences, Faculty of Physical Education and Health in Biała Podlaska, Józef Piłsudski University of Physical Education in Warsaw, 21-500 Biała Podlaska, Poland.
Department of Sports Sciences, Faculty of Physical Education and Health in Biała Podlaska, Józef Piłsudski University of Physical Education in Warsaw, 21-500 Biała Podlaska, Poland.
Int J Environ Res Public Health. 2022 Jul 2;19(13):8127. doi: 10.3390/ijerph19138127.
The aim of the study was to evaluate whether the most common polymorphisms in the and genes affect the circulating heat shock protein 70 (HSP70), as well as inflammatory and prooxidant-antioxidant parameters in healthy men undergoing chronic endurance training. The subjects were randomly assigned to a 12-week swimming training (ST group) or control group (CON). Fasting blood samples were collected pre- and post-study period to assessment: superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities, serum levels of lipid hydroperoxides (LHs), tumor necrosis factor α (TNFα), and HSP70. Subjects were genotyped for -174G/C, + 190 G/C and + 1538 A/G single nucleotide polymorphisms (SNPs) by real-time PCR. After a 12-week study period, a decrease in TNFα, HSP70, and GPx was observed in the ST group, but not the CON group. SNP affected serum TNFα levels (main effect of genotype). Higher TNFα levels (pre- and post-study period) was observed in CC CON than in other genotypes of CON and ST groups. However, a post-training decrease in TNFα was observed in both GG and CC genotypes of ST group. In turn, only GG genotype of the ST group was related to a post-training decrease in HSP70 (main time and genotype interaction). Moreover, pre- and post-training LHs were lower in GG than GC/CC genotypes of the ST group (main genotype effect). In conclusion, polymorphisms within the and genes seem to affect baseline levels of some inflammatory parameters and prooxidant-antioxidant status and/or their changes after chronic swimming training. However, the results should be confirmed in a study with a larger sample size, one that includes individuals with sedentary lifestyles.
本研究旨在评估 和 基因中最常见的多态性是否会影响健康男性在进行慢性耐力训练时的循环热休克蛋白 70（HSP70）以及炎症和促氧化剂-抗氧化剂参数。将受试者随机分为 12 周游泳训练（ST 组）或对照组（CON）。在研究前后采集空腹血样，以评估：超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）活性、血清脂质过氧化物（LH）水平、肿瘤坏死因子α（TNFα）和 HSP70。通过实时 PCR 对 -174G/C、+190G/C 和 +1538A/G 单核苷酸多态性（SNP）进行基因分型。经过 12 周的研究，ST 组 TNFα、HSP70 和 GPx 下降，但 CON 组无此变化。+190G/C SNP 影响血清 TNFα 水平（基因型的主要作用）。与 CON 和 ST 组的其他基因型相比，CON 组 CC 型的 TNFα 水平更高（研究前后）。然而，ST 组 GG 和 CC 型的 TNFα 在训练后均下降。相反，只有 ST 组的 GG 基因型与 HSP70 的训练后下降有关（时间和基因型的主要相互作用）。此外，ST 组的 GG 基因型在训练前后的 LH 均低于 GC/CC 基因型（主要基因型效应）。总之， 和 基因内的多态性似乎会影响某些炎症参数和促氧化剂-抗氧化剂状态的基线水平，以及慢性游泳训练后的变化。然而，这些结果需要在更大样本量的研究中得到证实，该研究应包括久坐生活方式的个体。
