Won Ran, Lee Kyung Hee, Lee Bae Hwan
Department of Biomedical Laboratory Science, Dongseo University, Busan, Korea.
Neuroreport. 2011 Oct 5;22(14):721-6. doi: 10.1097/WNR.0b013e32834acb8d.
This study investigated the neuroprotective effects of coenzyme Q10 (CoQ10) against oxidative stress induced by kainic acid (KA) in organotypic hippocampal slice culture of rats. Cultured slices were injured by exposure to 5 µM of KA for 18 h and then treated with different concentrations of CoQ10. Neuronal cell death measured as propidium iodide uptake was reduced at 24 h after treatment with 1 µM of CoQ10. We also observed an increased number of surviving CA3 neurons in 0.1 and 1 µM concentrations of CoQ10-treated groups using cresyl violet staining. CoQ10 (0.01, 0.1, and 1 µM) treatment significantly decreased the 2',7'-dichlorofluorescein fluorescence and the expression of NQO1 in the CoQ10-treated groups was significantly lower than that in the KA-only group. These results suggest that CoQ10 may protect hippocampal neurons against oxidative stress.
本研究调查了辅酶Q10（CoQ10）对大鼠海马脑片培养物中由海藻酸（KA）诱导的氧化应激的神经保护作用。将培养的脑片暴露于5µM的KA中18小时以造成损伤，然后用不同浓度的CoQ10进行处理。用碘化丙啶摄取量衡量的神经元细胞死亡在使用1µM CoQ10处理24小时后减少。我们还使用甲酚紫染色观察到，在0.1和1µM浓度的CoQ10处理组中，存活的CA3神经元数量增加。CoQ10（0.01、0.1和1µM）处理显著降低了2'，7'-二氯荧光素荧光，并且CoQ10处理组中NQO1的表达显著低于仅KA处理组。这些结果表明，CoQ10可能保护海马神经元免受氧化应激。
