α-生育三烯酚对海人酸诱导的器官型海马脑片培养神经毒性的神经保护作用。
Neuroprotective effects of α-tocotrienol on kainic acid-induced neurotoxicity in organotypic hippocampal slice cultures.
机构信息
Department of Physiology, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Korea.
出版信息
Int J Mol Sci. 2013 Sep 5;14(9):18256-68. doi: 10.3390/ijms140918256.
Vitamin E, such as alpha-tocopherol (ATPH) and alpha-tocotrienol (ATTN), is a chain-breaking antioxidant that prevents the chain propagation step during lipid peroxidation. In the present study, we investigated the effects of ATTN on KA-induced neuronal death using organotypic hippocampal slice culture (OHSC) and compared the neuroprotective effects of ATTN and ATPH. After 15 h KA (5 µM) treatment, delayed neuronal death was detected in the CA3 region and reactive oxygen species (ROS) formation and lipid peroxidation were also increased. Both co-treatment and post-treatment of ATPH (100 µM) or ATTN (100 µM) significantly increased the cell survival and reduced the number of TUNEL-positive cells in the CA3 region. Increased dichlorofluorescein (DCF) fluorescence and levels of thiobarbiturate reactive substances (TBARS) were decreased by ATPH and ATTN treatment. These data suggest that ATPH and ATTN treatment have protective effects on KA-induced cell death in OHSC. ATTN treatment tended to be more effective than ATPH treatment, even though there was no significant difference between ATPH and ATTN in co-treatment or post-treatment.
维生素 E，如α-生育酚（ATPH）和α-生育三烯酚（ATTN），是一种链断裂抗氧化剂，可防止脂质过氧化过程中的链传播步骤。在本研究中，我们使用器官型海马切片培养（OHSC）研究了 ATTN 对 KA 诱导的神经元死亡的影响，并比较了 ATTN 和 ATPH 的神经保护作用。在 KA（5 µM）处理 15 h 后，在 CA3 区检测到迟发性神经元死亡，并且还增加了活性氧（ROS）形成和脂质过氧化。ATP 和 ATT 共处理和后处理（100 µM）均显著增加了 CA3 区的细胞存活率并减少了 TUNEL 阳性细胞的数量。二氯荧光素（DCF）荧光的增加和硫代巴比妥酸反应物质（TBARS）的水平通过 ATPH 和 ATT 处理降低。这些数据表明，ATP 和 ATT 处理对 OHSC 中 KA 诱导的细胞死亡具有保护作用。ATT 处理的效果趋于优于 ATPH 处理，尽管在共处理或后处理中，ATP 和 ATT 之间没有显著差异。
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