Department of Dermatology, Second Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, China.
Int J Biol Sci. 2011;7(7):912-26. doi: 10.7150/ijbs.7.912. Epub 2011 Jul 31.
Stem cell transplantation is a promising strategy for delayed wound healing caused by chemotherapy. However, the fate of stem cells under chemotherapy has not been fully elucidated. Herein we characterized human fetal bone marrow stromal cells (hBMSCs) during wound healing in mice treated with cyclophosphamide (CTX). The isolated hBMSCs expressed the phenotype of CD11b(low)/CD14(low)/CD34(low)/CD45(low)/CD29(high)/CD44(high)/CD90(high)/CD105(high)/CD146(high)/STRO-1(low). Following in vitro exposure to CTX, hBMSCs showed decreased cell growth in a dose- and time-dependent manner, accompanied by increased expressions of collagen-I/III, and CD31. After transplantation, wounds closed as early as 8 days and were positive for α-smooth muscle actin (α-SMA), implicating the enhanced re-epithelialization and wound contraction. Moreover, proliferating cell nuclear antigen (PCNA) and CD31 showed co-localization with α-SMA, suggesting the differentiation of hBMSCs into epithelial cells and myofibroblasts/fibroblasts. Taken together, our results indicate hBMSCs can accelerate wound healing under chemotherapy through altering their phenotypes.
干细胞移植是一种有前途的策略，可用于治疗化疗引起的延迟性伤口愈合。然而，化疗下干细胞的命运尚未完全阐明。在此，我们在接受环磷酰胺（CTX）治疗的小鼠伤口愈合过程中对人胎骨髓基质细胞（hBMSCs）进行了特征描述。分离的 hBMSCs 表达 CD11b(low)/CD14(low)/CD34(low)/CD45(low)/CD29(high)/CD44(high)/CD90(high)/CD105(high)/CD146(high)/STRO-1(low) 的表型。在体外暴露于 CTX 后，hBMSCs 的细胞生长呈剂量和时间依赖性下降，同时胶原-I/III 和 CD31 的表达增加。移植后，伤口早在 8 天就闭合，并呈α-平滑肌肌动蛋白（α-SMA）阳性，提示上皮细胞和肌纤维母细胞/成纤维细胞的再上皮化和伤口收缩增强。此外，增殖细胞核抗原（PCNA）和 CD31 与 α-SMA 共定位，表明 hBMSCs 分化为上皮细胞和肌纤维母细胞/成纤维细胞。总之，我们的研究结果表明，hBMSCs 通过改变其表型可以加速化疗下的伤口愈合。
