Department of Oral Microbiology, Health Sciences University of Hokkaido Graduate School of Dentistry, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido 061-0293, Japan.
Curr Microbiol. 2011 Nov;63(5):403-7. doi: 10.1007/s00284-011-9993-2. Epub 2011 Aug 18.
Five species of oral Veillonella, V. atypica, V. denticariosi, V. dispar, V. parvula, and V. rogosae, have been suggested to be early colonizers of dental biofilm and causes of opportunistic infections and oral malodor. However, the pathogenicity and the distribution of oral Veillonella spp. have not been clarified. Previously, oral Veillonella spp. were identified by using 16S rDNA sequence analysis. In addition, recently, Veillonella isolates from human tongue biofilm were identified by rpoB gene sequences, but these procedures are time-consuming and complex. To overcome this problem, Igarashi et al. have designed species-specific primer sets for oral Veillonella spp. by using a highly variable region in the rpoB gene. In the present study, the distribution and frequency of oral Veillonella spp. in the tongue biofilm of healthy adults in their 20s were examined by using these species-specific primer sets. Tongue biofilms of these subjects were found to be divided into two groups based on the distribution and frequency of oral Veillonella spp. In one group, V. rogosae was the predominant species; the other group consisted of mainly V. dispar and V. atypica. Multiple factors may influence these differences in distribution and frequency of oral Veillonella spp. in tongue biofilm. This is the first report also demonstrating the availability of the species-specific primer sets for PCR to determine the distribution and frequency of oral Veillonella spp. in the tongue biofilm of healthy adults in their 20s.
五种口腔韦荣氏菌，即不典型韦荣氏菌、牙垢韦荣氏菌、差异韦荣氏菌、微小韦荣氏菌和罗格氏韦荣氏菌，被认为是牙菌斑生物膜的早期定植者，并引起机会性感染和口腔异味。然而，口腔韦荣氏菌的致病性和分布尚未明确。以前，口腔韦荣氏菌是通过 16S rDNA 序列分析来鉴定的。此外，最近，通过 rpoB 基因序列鉴定了来自人类舌生物膜的韦荣氏菌分离株，但这些程序耗时且复杂。为了解决这个问题，Igarashi 等人通过 rpoB 基因中的高度可变区设计了口腔韦荣氏菌的种特异性引物。在本研究中，使用这些种特异性引物检测了 20 多岁健康成年人舌生物膜中口腔韦荣氏菌的分布和频率。这些受试者的舌生物膜根据口腔韦荣氏菌的分布和频率分为两组。一组以罗格氏韦荣氏菌为主导种，另一组主要由差异韦荣氏菌和不典型韦荣氏菌组成。多种因素可能影响舌生物膜中口腔韦荣氏菌分布和频率的差异。这是首次报道也证明了种特异性引物用于 PCR 确定 20 多岁健康成年人舌生物膜中口腔韦荣氏菌的分布和频率是可行的。
