Kraatz Mareike, Taras David
Institute of Animal Nutrition, Faculty of Veterinary Medicine, Free University Berlin, Berlin, Germany.
Int J Syst Evol Microbiol. 2008 Dec;58(Pt 12):2755-61. doi: 10.1099/ijs.0.2008/001032-0.
A bacterium, designated strain lac18(T), was isolated in pure culture from the mucosal jejunum of a healthy pig, using a medium selective for anaerobic lactic acid bacteria and containing porcine gastric mucin as the main carbon and nitrogen source. Cells of this strain were coccus-shaped, arranged singly or in pairs and were Gram-stain-negative, oxidase-negative, non-spore-forming, anaerobic and microaerotolerant. An analysis based on 16S rRNA gene sequences indicated that strain lac18(T) should be assigned to the genus Veillonella, class Clostridia, phylum Firmicutes. 16S rRNA and dnaK gene sequence-based phylogenetic analyses both indicated that the most closely related species were Veillonella ratti ATCC 17746(T) (similarities of 96.6 and 84.5 %, respectively) and Veillonella criceti ATCC 17747(T) (similarities of 96.6 and 83.4 %, respectively). The results of DNA-DNA hybridizations between strain lac18(T) and these Veillonella species and the type species of the genus, Veillonella parvula DSM 2008(T), confirmed the genotypic distinctness of the novel isolate. Data from phenotypic studies also served to differentiate strain lac18(T) from related strains. Therefore strain lac18(T) represents a novel species of the genus Veillonella, for which the name Veillonella magna sp. nov. is proposed. The type strain is lac18(T) (=CCUG 55454(T)=CIP 109767(T)=DSM 19857(T)=JCM 15053(T)).
从一头健康猪的空肠黏膜中，使用一种对厌氧乳酸菌具有选择性且以猪胃黏蛋白作为主要碳源和氮源的培养基，分离得到一株纯培养细菌，命名为菌株lac18(T)。该菌株细胞呈球菌状，单个或成对排列，革兰氏染色阴性，氧化酶阴性，不产芽孢，厌氧且耐微氧。基于16S rRNA基因序列的分析表明，菌株lac18(T)应归属于梭菌纲、厚壁菌门的韦荣氏菌属。基于16S rRNA和dnaK基因序列的系统发育分析均表明，与其亲缘关系最近的物种是鼠韦荣氏菌ATCC 17746(T)（相似性分别为96.6%和84.5%）和仓鼠韦荣氏菌ATCC 17747(T)（相似性分别为96.6%和83.4%）。菌株lac18(T)与这些韦荣氏菌属物种以及该属模式种小韦荣氏菌DSM 2008(T)之间的DNA-DNA杂交结果，证实了该新分离菌株在基因型上的独特性。表型研究数据也有助于将菌株lac18(T)与相关菌株区分开来。因此，菌株lac18(T)代表韦荣氏菌属的一个新物种，为此提议将其命名为大韦荣氏菌（Veillonella magna sp. nov.）。模式菌株为lac18(T)（=CCUG 55454(T)=CIP 109767(T)=DSM 19857(T)=JCM 15053(T)）。
