Key Laboratory of Tea Biochemistry and Biotechnology, Ministry of Education and Ministry of Agriculture, Anhui Agriculture University, Hefei, China.
Plant Cell Rep. 2012 Jan;31(1):27-34. doi: 10.1007/s00299-011-1136-5. Epub 2011 Aug 18.
C-repeat/dehydration-responsive element binding factors (CBFs) can induce the expression of a suite of cold-responsive genes to increase plant cold tolerance, and inducer of CBF expression 1 (ICE1) is a major activator for CBF. In the present study, we isolated the full-length cDNAs of ICE1 and CBF from Camellia sinensis, designated as CsICE1 and CsCBF1, respectively. The deduced protein CsICE1 contains a highly conserved basic helix-loop-helix (bHLH) domain and C-terminal region of ICE1-like proteins. CsCBF1 contains all conserved domains of CBFs in other plant species and can specifically bind to the C-repeat/dehydration-responsive element (CRT/DRE) as confirmed by electrophoretic mobility shift assay. The transcription of CsICE1 had no apparent alteration after chilling treatment (4°C). CsCBF1 expression was not detected in normal temperature (20°C) but was induced immediately and significantly by low temperature (4°C). Our results suggest that ICE1-CBF cold-response pathway is conserved in tea plants. CsICE1 and CsCBF1, two components of this pathway, play roles in cold responses in tea plants.
C-重复/脱水响应元件结合因子（CBFs）可以诱导一系列冷响应基因的表达，从而提高植物的耐寒性，而 CBF 表达诱导因子 1（ICE1）是 CBF 的主要激活因子。在本研究中，我们从茶树中分离出 ICE1 和 CBF 的全长 cDNA，分别命名为 CsICE1 和 CsCBF1。推导的蛋白 CsICE1 含有高度保守的基本螺旋-环-螺旋（bHLH）结构域和 ICE1 样蛋白的 C 端区域。CsCBF1 含有其他植物物种 CBFs 的所有保守结构域，并能通过电泳迁移率变动分析特异性结合 C-重复/脱水响应元件（CRT/DRE）。低温处理（4°C）后，CsICE1 的转录没有明显改变。CsCBF1 在正常温度（20°C）下未检测到表达，但立即并显著被低温（4°C）诱导。我们的结果表明，ICE1-CBF 冷响应途径在茶树中是保守的。该途径的两个组成部分 CsICE1 和 CsCBF1 在茶树的冷响应中发挥作用。
