Sir William Dunn School of Pathology, South Parks Road, Oxford, OX1 3RE, UK.
Curr Opin Chem Biol. 2011 Oct;15(5):614-9. doi: 10.1016/j.cbpa.2011.07.017. Epub 2011 Aug 16.
The archaeal DNA replication apparatus is a simplified version of that of eukaryotes and has attracted attention as a tractable model system for the orthologous, but significantly more complex eukaryal machinery. A variety of archaeal model organisms have been investigated with strong emphasis on structural and biochemical analyses of replication-associated proteins. In this review we will describe recent advances in understanding the properties of the replicative helicase, the MCM complex, and the role of the sliding clamp, PCNA, in mediating a range of protein-DNA transactions. Although both complexes form ring shaped assemblies, they play very distinct roles at the leading and trailing edges of the replication fork machinery respectively.
古菌 DNA 复制装置是真核生物的简化版本，作为同源但复杂得多的真核机器的可行模型系统而受到关注。已经研究了多种古菌模式生物，重点是复制相关蛋白的结构和生化分析。在这篇综述中，我们将描述在理解复制解旋酶、MCM 复合物的性质以及滑动夹 PCNA 在介导一系列蛋白-DNA 相互作用方面的作用方面的最新进展。尽管这两个复合物都形成环状组装，但它们在复制叉机器的前导和尾随边缘分别发挥着非常不同的作用。
