Hartley T M, Khabbaz R F, Cannon R O, Kaplan J E, Lairmore M D
Retrovirus Diseases Branch, Centers for Disease Control, Atlanta, Georgia 30333.
J Clin Microbiol. 1990 Apr;28(4):646-50. doi: 10.1128/jcm.28.4.646-650.1990.
We have characterized the immunoreactivity to human T-cell lymphotropic virus type I (HTLV-I) among 26,983 persons of various seroprevalence groups by using enzyme immunoassay, immunoblot (IB), and radioimmunoprecipitation assays (RIPA) in accordance with Public Health Service recommended guidelines for the interpretation of serologic test results for HTLV-I infection. IB-indeterminate serum specimens (n = 178) were reactive to HTLV-I gag proteins, and no serum contained only env reactivity. Overall, RIPA resolved 40% of IB-indeterminate serum samples; however, the probability that RIPA would confirm IB-indeterminate samples depended on the seroprevalence of the population tested. HTLV-I gag p19-only reactivity on IB was not a reliable marker of HTLV-I infection, while gag p24 reactivity on IB was clearly associated with positive seroreactive specimens. IB and RIPA tests did not clearly distinguish between HTLV-I and HTLV-II seroreactivities. These data emphasize that patterns of immunoreactivity to HTLV-I antigens are dependent upon the seroprevalence of the risk groups tested. In addition, RIPA detected antibodies to env proteins present in low titer in a substantial number of IB gag-only reactive sera and resolved the HTLV-I antibody status of these sera.
我们按照公共卫生服务部门推荐的用于解释人类嗜T淋巴细胞病毒I型（HTLV-I）感染血清学检测结果的指南，通过酶免疫测定、免疫印迹（IB）和放射免疫沉淀测定（RIPA），对26983名不同血清阳性率组的人群进行了HTLV-I免疫反应性特征分析。IB检测结果不确定的血清标本（n = 178）对HTLV-I gag蛋白呈反应性，且没有血清仅呈现env反应性。总体而言，RIPA解决了40%的IB检测结果不确定的血清样本；然而，RIPA确认IB检测结果不确定样本的概率取决于所检测人群的血清阳性率。IB上仅出现HTLV-I gag p19反应性并非HTLV-I感染的可靠标志物，而IB上的gag p24反应性则明显与血清反应阳性标本相关。IB和RIPA检测未能明确区分HTLV-I和HTLV-II的血清反应性。这些数据强调，对HTLV-I抗原的免疫反应模式取决于所检测风险组的血清阳性率。此外，RIPA在大量仅对IB gag呈反应性的血清中检测到了低滴度存在的env蛋白抗体，并明确了这些血清的HTLV-I抗体状态。
