使用超分子凝胶电泳分离蛋白质。
Separation of proteins using supramolecular gel electrophoresis.
机构信息
Department of Chemistry, Faculty of Science, Shizuoka University, 836 Ohya, Shizuoka 422-8529, Japan.
出版信息
Chem Commun (Camb). 2011 Oct 7;47(37):10344-6. doi: 10.1039/c1cc13826j. Epub 2011 Aug 19.
PMID:21853178
Abstract
An amphiphilic low-molecular-weight hydrogelator 1 was synthesized. A tris-glycine-SDS solution gel of 1 was applied for electrophoresis to separate proteins. Centrifugation of a mixture of protein and a hydrogel of 1 enabled the recovery of protein. Various combinations of proteins were applied for supramolecular gel electrophoresis (SUGE), and remarkably poor mobility for small proteins (<45 kDa) was found.
摘要
合成了一种两亲性的低分子量水凝胶因子 1。1 的三甘氨酸-SDS 溶液凝胶被用于电泳分离蛋白质。将蛋白质和 1 的水凝胶混合物进行离心，可实现蛋白质的回收。我们将各种蛋白质组合用于超分子凝胶电泳（SUGE），发现小分子蛋白质（<45 kDa）的迁移率非常低。
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