Kröger M, Wahl R, Rice P
Institut für Mikrobiologie und Molekularbiologie, Justus-Liebig-Universität Giessen, FRG.
Nucleic Acids Res. 1990 Apr 25;18 Suppl(Suppl):2549-87. doi: 10.1093/nar/18.suppl.2549.
We have compiled the DNA sequence data for E.coli available from the GENBANK and EMBL data libraries and over a period of several years independently from the literature. This is the second listing replacing and increasing the former listing roughly by one third. After deletion of all detected overlaps a total of 1 248 696 individual bp is found to be determined till the beginning of 1990. This corresponds to a total of 26.46% of the entire E. coli chromosome consisting of about 4,720 kbp. This number may actually be higher by some extra 2% derived from the sequence of lysogenic bacteriophage lambda and various insertion sequences. This compilation is now available in machine readable form from the EMBL data library.
我们从GENBANK和EMBL数据库中搜集了多年来独立于文献的大肠杆菌DNA序列数据。这是第二次列表,取代了之前的列表并使其数量大致增加了三分之一。在删除所有检测到的重叠部分后,到1990年初共确定了1248696个单独的碱基对。这相当于整个大肠杆菌染色体(约4720kbp)的26.46%。实际上,这个数字可能还会因溶原性噬菌体λ和各种插入序列的序列而高出约2%。这个汇编现在可以从EMBL数据库以机器可读的形式获取。