Kröger M
Institut für Mikrobiologie und Molekularbiologie, Fachbereich Biologie, Justus-Liebig-Universität Giessen, FRG.
Nucleic Acids Res. 1989;17 Suppl(Suppl):r283-309. doi: 10.1093/nar/17.suppl.r283.
We have compiled the DNA sequence data for E. coli K12 available from the GENBANK and EMBO databases and over a period of several years independently from the literature. We have introduced all available genetic map data and have arranged the sequences accordingly. As far as possible the overlaps are deleted and a total of 940,449 individual bp is found to be determined till the beginning of 1989. This corresponds to a total of 19.92% of the entire E. coli chromosome consisting of about 4,720 kbp. This number may actually be higher by some extra 2% derived from the sequence of lysogenic bacteriophage lambda and the various insertion sequences. This compilation may be available in machine readable form from one of the international databanks in some future.
我们从GENBANK和EMBO数据库收集了大肠杆菌K12的DNA序列数据,这些数据是在几年时间里独立于文献收集的。我们引入了所有可用的遗传图谱数据,并据此排列了序列。尽可能删除了重叠部分,到1989年初共确定了940,449个独立碱基对。这相当于整个大肠杆菌染色体(约4,720 kbp)的19.92%。实际上,这个数字可能会因溶原性噬菌体λ的序列和各种插入序列而高出约2%。这个汇编在未来某个时候可能会以机器可读的形式从某个国际数据库获得。