Department of Drug Sciences, G. d' Annunzio University, via dei Vestini 31, 66013 Chieti, Italy.
Peptides. 2011 Sep;32(9):1866-71. doi: 10.1016/j.peptides.2011.08.003. Epub 2011 Aug 9.
Visceral adipose tissue-derived serpin (vaspin) improves glucose tolerance and insulin sensitivity in diet-induced obese mice. Chemerin may increase insulin sensitivity in adipose tissue and seems to be associated with several key aspects of metabolic syndrome. Decreased levels of omentin-1 are associated with increasing obesity and insulin resistance. Our study aimed to investigate the effects of vaspin, chemerin and omentin-1 acute administration on feeding and hypothalamic gene expression of peptides which play a key role in feeding regulation. 35 rats were injected into the arcuate nucleus (ARC) of the hypothalamus with either saline (n=8), vaspin (1μg/kg; n=9), chemerin (8μg/kg; n=9), or omentin-1 (8μg/kg; n=9). Food intake in the following 24h was recorded, thereafter rats were sacrificed. Total RNA was extracted from hypothalami and reverse transcribed to evaluate hypothalamic gene expression of agouti-related peptide (AgRP), neuropeptide Y (NPY), orexin-A, cocaine- and amphetamine-regulated transcript (CART), corticotrophin releasing hormone (CRH) and proopiomelanocortin (POMC), by real-time reverse transcription polymerase chain reaction. Compared to vehicle, vaspin injection significantly decreased feeding, while chemerin and omentin-1 had no effect in the tested dose. Vaspin treatment significantly decreased NPY and increased POMC gene expression. Chemerin treatment led to a significant increase of both AgRP and POMC gene expression. Omentin-1 treatment did not modify gene expression of the investigated peptides. Therefore, vaspin is an adipokine triggering anorectic pathways in the hypothalamus, where reduction of NPY and increase of POMC mRNA levels mediate feeding inhibition. Chemerin and omentin-1 have no effect on feeding in the tested dose.
内脏脂肪组织来源的丝氨酸蛋白酶抑制剂（vaspin）可改善饮食诱导肥胖小鼠的葡萄糖耐量和胰岛素敏感性。趋化素可能会增加脂肪组织的胰岛素敏感性，并且似乎与代谢综合征的几个关键方面有关。网膜素-1水平降低与肥胖和胰岛素抵抗的增加有关。我们的研究旨在探讨 vaspin、趋化素和网膜素-1 急性给药对摄食和参与摄食调节的下丘脑肽基因表达的影响。35 只大鼠被注射到下丘脑弓状核（ARC）中，分别注射生理盐水（n=8）、vaspin（1μg/kg；n=9）、趋化素（8μg/kg；n=9）或网膜素-1（8μg/kg；n=9）。记录随后 24 小时的食物摄入量，然后处死大鼠。从下丘脑提取总 RNA，反转录以评估下丘脑 AgRP、NPY、orexin-A、可卡因和安非他命调节转录物（CART）、促肾上腺皮质激素释放激素（CRH）和 proopiomelanocortin（POMC）的基因表达，实时逆转录聚合酶链反应。与载体相比，vaspin 注射显著减少摄食，而趋化素和网膜素-1 在测试剂量下没有作用。Vaspin 处理显著降低 NPY 并增加 POMC 基因表达。趋化素处理导致 AgRP 和 POMC 基因表达均显著增加。网膜素-1 处理没有改变所研究肽的基因表达。因此，vaspin 是一种脂肪因子，可触发下丘脑的厌食途径，其中 NPY 的减少和 POMC mRNA 水平的增加介导摄食抑制。在测试剂量下，趋化素和网膜素-1 对摄食没有影响。
