一种新型蛋白质组学生物标志物谱作为卵巢癌患者的诊断工具。

A novel proteomic biomarker panel as a diagnostic tool for patients with ovarian cancer.

机构信息

University of Copenhagen, Copenhagen, Denmark.

出版信息

Gynecol Oncol. 2011 Nov;123(2):308-13. doi: 10.1016/j.ygyno.2011.07.018. Epub 2011 Aug 19.

DOI:10.1016/j.ygyno.2011.07.018

PMID:21855971

Abstract

BACKGROUND

Previous reports have shown that the proteomic markers apolipoprotein A1, hepcidin, transferrin, inter-alpha trypsin IV internal fragment, transthyretin, connective-tissue activating protein 3 and beta-2 microglobulin may discriminate between a benign pelvic mass and ovarian cancer (OC). The aim was to determine if these serum proteomic biomarkers alone as well as in combination with age and serum CA125, could be helpful in triage of women with a pelvic mass.

METHODS

We included prospectively 144 patients diagnosed with (OC), 40 with a borderline tumor and 469 with a benign tumor. Surface-enhanced laser desorption/ionization time of flight-mass spectrometry was used for analyses. The Danish Index (DK-Index) based on the proteomic data, age and CA125 was developed using logistic regression models.

RESULTS

Multivariate logistic regression analysis demonstrated that the selected proteomic markers, CA125 and age were independent predictors of OC and the combination of these is proposed as the DK-index. A sensitivity (SN) of 99% had a specificity (SP) of 57% for DK-index and 49% for CA125. At a SN of 95%, the SP increased to 81% for DK-index compared to 68% for CA125 alone. For stage I+II the SP was 58% for DK-index and 49% for CA125. For stage III+IV the corresponding values were 94% and 86% respectively.

CONCLUSIONS

The DK-index warrants further evaluation in independent cohorts.

摘要

背景

先前的报告表明，载脂蛋白 A1、铁调素、转铁蛋白、α-胰蛋白酶 IV 内片段、转甲状腺素蛋白、结缔组织激活蛋白 3 和β-2 微球蛋白等蛋白质组学标志物可区分良性盆腔肿块和卵巢癌（OC）。本研究旨在确定这些血清蛋白质组学生物标志物是否可单独以及与年龄和血清 CA125 联合用于盆腔肿块患者的分诊。

方法

我们前瞻性地纳入了 144 例被诊断为 OC 的患者、40 例交界性肿瘤患者和 469 例良性肿瘤患者。使用表面增强激光解吸/电离飞行时间质谱分析。使用逻辑回归模型基于蛋白质组学数据、年龄和 CA125 开发丹麦指数（DK-Index）。

结果

多变量逻辑回归分析表明，所选蛋白质组学标志物、CA125 和年龄是 OC 的独立预测因子，这些标志物的组合被提出作为 DK-Index。DK-Index 的敏感性（SN）为 99%，特异性（SP）为 57%，CA125 的 SN 为 49%。当 SN 为 95%时，与 CA125 相比，DK-Index 的 SP 增加至 81%。对于 I+II 期，DK-Index 的 SP 为 58%，CA125 的 SP 为 49%。对于 III+IV 期，相应的值分别为 94%和 86%。