Department of Chemical Engineering, Pohang University of Science and Technology, San 31, Hyoja-Dong, Pohang 790-784, Republic of Korea.
Gyeongbuk Sea Grant Institute, Pohang University of Science and Technology, San 31, Hyoja-Dong, Pohang 790-784, Republic of Korea.
Int J Syst Evol Microbiol. 2012 Jul;62(Pt 7):1528-1535. doi: 10.1099/ijs.0.031302-0. Epub 2011 Aug 19.
A Gram-staining-negative, strictly aerobic, non-motile, yellow-pigmented bacterium, designated strain M091(T), was isolated from seawater at Damupo beach in Pohang, Republic of Korea, and investigated using a polyphasic taxonomic approach. The novel strain grew optimally at 25 °C, pH 7.0-8.0, and in the presence of 3% (w/v) NaCl. In a phylogenetic analysis based on 16S rRNA gene sequences, strain M091(T) formed a lineage within the family Flavobacteriaceae that was distinct from the most closely related genera of Flaviramulus (95.1% sequence similarity), Algibacter (94.9-93.9%), Mariniflexile (94.8-94.2%), Winogradskyella (94.8-93.2%), Lacinutrix (94.7-93.8%) and Tamlana (94.7-92.9%). The polar lipid profile of the novel strain comprised phosphatidylethanolamine, two unidentified aminolipids, one unidentified phospholipid and seven unidentified lipids. The predominant cellular fatty acids were iso-C(15:0) (20.5%), iso-C(17:0) 3-OH (15.4%), iso-C(15:0) 3-OH (12.4%), C(15:0) (10.9%) and iso-C(15:1) G (9.9%). The genomic DNA G+C content of strain M091(T) was 34.4 mol% and the major respiratory quinone was MK-6. Based on phenotypic and genotypic data, strain M091(T) represents a new genus and novel species in the family Flavobacteriaceae, for which the name Postechiella marina gen. nov., sp. nov. is proposed. The type strain of the type species is M091(T) (=KCTC 23537(T)=JCM 17630(T)).
一株革兰氏染色阴性、严格需氧、非运动、黄色素产生的细菌，被命名为 M091(T) 菌株，从韩国浦项市 Damupo 海滩的海水中分离得到，并通过多相分类学方法进行了研究。该新菌株在 25°C、pH7.0-8.0 和 3%（w/v）NaCl 的存在下生长最佳。在基于 16S rRNA 基因序列的系统发育分析中，M091(T) 菌株形成了在黄杆菌科内与最密切相关的属（Flaviramulus，95.1%序列相似性）、Algibacter（94.9-93.9%）、Mariniflexile（94.8-94.2%）、Winogradskyella（94.8-93.2%）、Lacinutrix（94.7-93.8%）和 Tamlana（94.7-92.9%）区别明显的谱系。新菌株的极性脂谱包括磷脂酰乙醇胺、两种未鉴定的胺脂、一种未鉴定的磷脂和七种未鉴定的脂类。主要的细胞脂肪酸为 iso-C(15:0)（20.5%）、iso-C(17:0) 3-OH（15.4%）、iso-C(15:0) 3-OH（12.4%）、C(15:0)（10.9%）和 iso-C(15:1) G（9.9%）。M091(T) 菌株的基因组 DNA G+C 含量为 34.4mol%，主要的呼吸醌为 MK-6。基于表型和基因型数据，M091(T) 菌株代表了黄杆菌科中的一个新属和新种，建议将其命名为 Postechiella marina 属，nov.，sp. nov.。模式种为 M091(T)（=KCTC 23537(T)=JCM 17630(T)）。
