RIKEN Omics Science Center, Yokohama, Kanagawa, Japan.
PLoS One. 2011;6(8):e23385. doi: 10.1371/journal.pone.0023385. Epub 2011 Aug 15.
Human DICER1 protein cleaves double-stranded RNA into small sizes, a crucial step in production of single-stranded RNAs which are mediating factors of cytoplasmic RNA interference. Here, we clearly demonstrate that human DICER1 protein localizes not only to the cytoplasm but also to the nucleoplasm. We also find that human DICER1 protein associates with the NUP153 protein, one component of the nuclear pore complex. This association is detected predominantly in the cytoplasm but is also clearly distinguishable at the nuclear periphery. Additional characterization of the NUP153-DICER1 association suggests NUP153 plays a crucial role in the nuclear localization of the DICER1 protein.
人 DICER1 蛋白将双链 RNA 切割成小片段，这是产生单链 RNA 的关键步骤，单链 RNA 是细胞质 RNA 干扰的介导因子。在这里，我们清楚地表明，人 DICER1 蛋白不仅定位于细胞质，而且还定位于核质。我们还发现人 DICER1 蛋白与核孔复合体的一个组成部分 NUP153 蛋白相互作用。这种关联主要在细胞质中检测到，但在核周也能明显区分。对 NUP153-DICER1 相互作用的进一步表征表明，NUP153 在 DICER1 蛋白的核定位中起着关键作用。
