Department of Marine & Coastal Studies, Madurai Kamaraj University, Madurai-625021, Tamilnadu, India.
Toxicol Mech Methods. 2012 Feb;22(2):111-7. doi: 10.3109/15376516.2011.603393. Epub 2011 Aug 23.
Profenofos (EC(50)) is a persistent and toxic organophosphorus insecticide. Animals get exposed to profenofos via food and water. The present study was designed to explore the genotoxic effect of profenofos in the marine fish. The ubiquitously occurring marine fish, Therapon jarbua, was exposed to profenofos and its effect on DNA was measured using comet (single-cell gel electrophoresis) assay. DNA damage were scored using mean percentage of tail length and compared with the comet classes' viz., 0, 1, 2, 3, and 4. In the first three doses, the (21.5, 43.0 and 86.0 µg L(-1)) comets were observed, of which the mean tail length differed significantly (p < 0.01) from those of unexposed, but not from each other. The mean tail length values were significantly (p < 0.05) higher in gill than in matured erythrocytes. The result indicates that DNA strand breaks in T. jarbua were due to the genotoxic potential of profenofos. From the study, we suggest that T. jarbua may be used as an indicator organism to assess the genotoxic risks of profenofos contamination in marine environments using Comet assay as an identification tool. We infer that organophosphorus insecticides may be dangerous to the marine lives.
丙溴磷（EC（50））是一种持久性和有毒的有机磷杀虫剂。动物通过食物和水接触到丙溴磷。本研究旨在探索丙溴磷对海洋鱼类的遗传毒性作用。本研究选择了广泛分布的海洋鱼类，褐牙鲆（Therapon jarbua），暴露于丙溴磷中，并使用彗星（单细胞凝胶电泳）试验测定其对 DNA 的影响。使用平均尾长的百分比对 DNA 损伤进行评分，并与彗星分类进行比较，即 0、1、2、3 和 4。在前三个剂量（21.5、43.0 和 86.0 µg L(-1))中观察到彗星，其中平均尾长与未暴露的彗星显著不同（p < 0.01），但彼此之间没有差异。在鳃中比在成熟红细胞中的平均尾长值显著更高（p < 0.05）。结果表明，丙溴磷的遗传毒性潜力导致了 T. jarbua 的 DNA 链断裂。从这项研究中，我们建议可以使用褐牙鲆作为指示生物，使用彗星试验作为鉴定工具，评估海洋环境中丙溴磷污染的遗传毒性风险。我们推断有机磷杀虫剂可能对海洋生物有危险。
