National Bureau of Fish Genetic Resources, Indian Council of Agricultural Research, Lucknow, India.
Mutat Res. 2011 Dec 24;726(2):209-14. doi: 10.1016/j.mrgentox.2011.09.011. Epub 2011 Sep 25.
The aim of the present study was to evaluate the induced genotoxicity (DNA damage) due to organophosphate pesticide profenofos (PFF) in gill cells of freshwater fish Channa punctatus using single cell gel electrophoresis (SCGE)/Comet assay. The 96h LC(50) value of PFF (50% EC) was estimated for the fish species in a semistatic system and then three sub-lethal of LC(50) concentrations viz the sub-lethal 1, sub-lethal 2 and sub-lethal 3 concentrations were determined as 0.58ppb, 1.16ppb and 1.74ppb, respectively. The fish specimens were exposed to these concentrations of the pesticide and the gill tissue samplings were done on 24h, 48h, 72h and 96h post exposure for assessment of DNA damage in terms of percentage of DNA in comet tails. In general, a concentration dependent response was observed in the gill cells with induction of maximum DNA damage at the highest concentration of PFF. The results of the present investigation indicated that PFF could potentially induce genotoxic effect in fish, even in sub-lethal concentrations and SCGE as a sensitive and reliable tool for in vivo assessment of DNA damage caused by the genotoxic agents.
本研究旨在利用单细胞凝胶电泳（SCGE）/彗星试验评估有机磷农药丙溴磷（PFF）对淡水鱼类 Channa punctatus 鳃细胞的诱导遗传毒性（DNA 损伤）。采用半静态系统测定 PFF（50% EC）对鱼类的 96hLC50 值，然后确定三个亚致死 LC50 浓度，即亚致死 1、亚致死 2 和亚致死 3 浓度分别为 0.58ppb、1.16ppb 和 1.74ppb。将鱼类标本暴露于这些农药浓度下，并在暴露后 24h、48h、72h 和 96h 进行鳃组织取样，以彗星试验中尾部 DNA 的百分比评估 DNA 损伤。一般来说，在鳃细胞中观察到浓度依赖性反应，在最高浓度的 PFF 下诱导最大的 DNA 损伤。本研究结果表明，PFF 甚至在亚致死浓度下也可能对鱼类产生遗传毒性效应，SCGE 是评估遗传毒性剂引起的 DNA 损伤的敏感可靠工具。
