Vijayan Sri Ramkumar, Santhiyagu Prakash, Singamuthu Muthukkumarasamy, Kumari Ahila Natarajan, Jayaraman Ravindran, Ethiraj Kannapiran
Department of Oceanography and Coastal Area Studies, School of Marine Sciences, Alagappa University, Thondi Campus, Thondi, Tamil Nadu 623 409, India.
SRM Research Institute, SRM University, Kattankulathur, Kancheepuram, Tamil Nadu 603 203, India.
ScientificWorldJournal. 2014 Feb 3;2014:938272. doi: 10.1155/2014/938272. eCollection 2014.
Silver and gold nanoparticles were synthesized using an aqueous extract of the seaweed Turbinaria conoides and their antibiofilm activity against marine biofilm forming bacteria is reported here. The UV-Vis spectra showed the characteristics SPR absorption band for Ag NPs at 421 and for Au NPs at 538 nm. Further, the synthesized nanoparticles were characterized using FT-IR, XRD, FESEM, EDX, and HRTEM analysis. Spherical and triangular nanostructures of the Ag and Au nanoparticles were observed between the size ranges of 2-17 nm and 2-19 nm, respectively. The synthesized Ag NPs are efficient in controlling the bacterial biofilm formation; however, Au NPs did not show any remarkable antibiofilm activity. The maximum zone of inhibition was recorded against E. coli (17.6 ± 0.42 mm), followed by Salmonella sp., S. liquefaciens, and A. hydrophila. The macrotube dilution method inferred the MIC (20-40 µL mL(-1)) and MBC (40-60 µL mL(-1)) of Ag NPs. The CLSM images clearly showed the weak adherence and disintegrating biofilm formation of marine biofilm bacterial strains treated with Ag NPs. The Artemia cytotoxicity assay recorded the LC50 value of 88.914 ± 5.04 µL mL(-1). Thus the present study proved the efficiency of Ag NPs as a potent antimicrofouling agent and became the future perspective for the possible usage in the biofouling related issues in the aquaculture installations and other marine systems.
使用海藻锥状旋链藻的水提取物合成了银和金纳米颗粒，并在此报告了它们对形成海洋生物膜的细菌的抗生物膜活性。紫外可见光谱显示，银纳米颗粒在421nm处有特征性表面等离子体共振吸收带，金纳米颗粒在538nm处有特征性表面等离子体共振吸收带。此外，使用傅里叶变换红外光谱、X射线衍射、场发射扫描电子显微镜、能谱分析和高分辨率透射电子显微镜分析对合成的纳米颗粒进行了表征。分别观察到银和金纳米颗粒的球形和三角形纳米结构，尺寸范围分别为2-17nm和2-19nm。合成的银纳米颗粒在控制细菌生物膜形成方面很有效；然而，金纳米颗粒没有显示出任何显著的抗生物膜活性。对大肠杆菌的抑制圈最大（17.6±0.42mm），其次是沙门氏菌、液化沙雷氏菌和嗜水气单胞菌。大试管稀释法推断出银纳米颗粒的最低抑菌浓度（20-40μL mL⁻¹）和最低杀菌浓度（40-60μL mL⁻¹）。共聚焦激光扫描显微镜图像清楚地显示了用银纳米颗粒处理的海洋生物膜细菌菌株的弱粘附和生物膜解体形成。卤虫细胞毒性试验记录的半数致死浓度值为88.914±5.04μL mL⁻¹。因此，本研究证明了银纳米颗粒作为一种有效的抗微生物污损剂的有效性，并成为其在水产养殖设施和其他海洋系统中与生物污损相关问题中可能应用的未来前景。
