Schröder D, Hehmke B, Klöting I, Besch W, Schmidt S
Central Institute of Diabetes, Karlsburg/GDR.
Exp Clin Endocrinol. 1990 Feb;95(1):22-30. doi: 10.1055/s-0029-1210930.
The present study investigates whether sera from diabetic BB/OK rats impair beta-cell-specific functions of rat pancreatic islets and whether humoral-mediated cytotoxicity in BB rat sera also affects the autologous islets of normoglycaemic serum donors. Exposure of neonatal rat islets of Langerhans for 20 h to sera from diabetic BB rats and rabbit complement resulted in a decrease in insulin content and in insulin release upon glucose stimulation. However, the extent of islet alterations caused by sera from various BB rats was different as revealed by comparison of the ability of beta-cells to restore their functions during a 48 h recovery period. While the effect of some of the sera was completely reversible, in other cases the insulin content and/or the secretory capacity of the islets could not be fully restored indicating partially irreversible islet alterations due to pretreatment with cytotoxic BB rat serum. The potential effect of anti-islet cytotoxicity in BB rat sera against autologous islets of the serum donor indicated by enhanced 51Cr-release in the presence of rabbit complement was proven in 50% (6/12) of the investigated normoglycaemic BB/OK rats. These results suggest that humoral-mediated cytotoxicity may contribute to beta-cell alteration in this model of diabetes.
本研究调查了糖尿病BB/OK大鼠的血清是否会损害大鼠胰岛的β细胞特异性功能，以及BB大鼠血清中的体液介导细胞毒性是否也会影响血糖正常血清供体的自体胰岛。将新生大鼠胰岛暴露于糖尿病BB大鼠的血清和兔补体中20小时，会导致胰岛素含量降低以及葡萄糖刺激后胰岛素释放减少。然而，通过比较β细胞在48小时恢复期内恢复其功能的能力发现，不同BB大鼠的血清引起的胰岛改变程度有所不同。虽然某些血清的作用是完全可逆的，但在其他情况下，胰岛的胰岛素含量和/或分泌能力无法完全恢复，这表明由于用细胞毒性BB大鼠血清预处理导致胰岛改变部分不可逆。在50%（6/12）的被研究血糖正常的BB/OK大鼠中，证实了BB大鼠血清中抗胰岛细胞毒性对血清供体自体胰岛的潜在影响，这表现为在兔补体存在下51Cr释放增强。这些结果表明，体液介导的细胞毒性可能在该糖尿病模型中导致β细胞改变。
