Burin Vívian Maria, Arcari Stefany Grützmann, Costa Léa Luzia Freitas, Bordignon-Luiz Marilde T
Departamento de Ciência e Tecnologia de Alimentos CAL/CCA, Florianópolis, SC, Brazil.
J Chromatogr Sci. 2011 Sep;49(8):647-51. doi: 10.1093/chrsci/49.8.647.
A methodology employing reversed-phase high-performance liquid chromatography (RP-HPLC) was developed and validated for simultaneous determination of five phenolic compounds in red wine. The chromatographic separation was carried out in a C(18) column with water acidify with acetic acid (pH 2.6) (solvent A) and 20% solvent A and 80% acetonitrile (solvent B) as the mobile phase. The validation parameters included: selectivity, linearity, range, limits of detection and quantitation, precision and accuracy, using an internal standard. All calibration curves were linear (R(2) > 0.999) within the range, and good precision (RSD < 2.6%) and recovery (80-120%) was obtained for all compounds. This method was applied to quantify phenolics in red wine samples from Santa Catarina State, Brazil, and good separation peaks for phenolic compounds in these wines were observed.
开发并验证了一种采用反相高效液相色谱法（RP-HPLC）同时测定红酒中五种酚类化合物的方法。色谱分离在C(18)柱上进行，以用乙酸酸化的水（pH 2.6）（溶剂A）和20%溶剂A与80%乙腈（溶剂B）作为流动相。验证参数包括：选择性、线性、范围、检测限和定量限、精密度和准确度，使用内标物。所有校准曲线在该范围内均呈线性（R(2) > 0.999），并且所有化合物均获得了良好的精密度（RSD < 2.6%）和回收率（80 - 120%）。该方法应用于定量分析巴西圣卡塔琳娜州红酒样品中的酚类物质，观察到这些红酒中酚类化合物的分离峰良好。
