Manasa Vallamkondu, Chaudhari Sachin R, Tumaney Ajay W
Academy of Scientific and Innovative Research (AcSIR) Ghaziabad - 201002 India.
Department of Lipid Science, Council of Scientific and Industrial Research - Central Food Technological Research Institute Mysore - 570 020 India
RSC Adv. 2020 Dec 11;10(72):43975-43984. doi: 10.1039/d0ra07794a. eCollection 2020 Dec 9.
γ-Oryzanol is an important group of nutraceuticals that play a key role in addressing metabolic disorders. This study, for the first time, examined volatile-free spice fixed oils (FOs) as an alternate plant source for γ-oryzanol and other nutraceuticals (phenolics, flavonoids, phytosterols, and tocopherols) using HPLC, HR-MS and NMR. The antioxidant activities of FOs were also analysed. The selected spices were , , , and . The major polyphenols and flavonoids quantified were gallic, protocatechuic, vanillic, syringic, -coumaric, ferulic, rutin, -cinnamic, and quercetin. FOs recorded high levels of ergosterol (48.56 mg/100 g) and stigmasterol (247.36 mg/100 g). The fucosterol levels were high in (268.31 mg/100 g) FOs, whereas FOs showed high content of β-sitosterols (7037.77 mg/100 g). and FOs recorded high α-tocopherol content (47.55 and 15.96 mg/100 g respectively). FOs showed high levels of three ferulates, namely, cycloartenyl ferulate, 24-methylene cycloartenyl ferulate and β-sitosteryl ferulate, whose contents were 89.42, 170.23 and 50.23 mg/100 g respectively which was confirmed by HRMS with a molecular mass (/) of 601.45, 615.47, and 589.45 respectively. Further, γ-oryzanol ferulates in FOs were confirmed by H-NMR analysis. The acidified methanolic extractives of FOs showed high free radical scavenging activity and antioxidant potential. These spice FOs have excellent antioxidant activities, and are novel potential functional ingredients against lifestyle disorders.
γ-谷维素是一类重要的营养保健品，在解决代谢紊乱问题中发挥着关键作用。本研究首次使用高效液相色谱法（HPLC）、高分辨质谱法（HR-MS）和核磁共振法（NMR），将无挥发性香料固定油（FOs）作为γ-谷维素和其他营养保健品（酚类、黄酮类、植物甾醇和生育酚）的替代植物来源进行了研究。还分析了固定油的抗氧化活性。所选香料为[此处原文缺失具体香料名称]。定量的主要多酚和黄酮类物质有没食子酸、原儿茶酸、香草酸、丁香酸、对香豆酸、阿魏酸、芦丁、反式肉桂酸和槲皮素。固定油中麦角甾醇（48.56毫克/100克）和豆甾醇（247.36毫克/100克）含量较高。岩藻甾醇含量在[此处原文缺失具体香料名称]固定油中较高（268.31毫克/100克），而[此处原文缺失具体香料名称]固定油中β-谷甾醇含量较高（7037.77毫克/100克）。[此处原文缺失具体香料名称]和[此处原文缺失具体香料名称]固定油的α-生育酚含量较高（分别为47.55和15.96毫克/100克）。[此处原文缺失具体香料名称]固定油中三种阿魏酸酯含量较高，即环阿屯醇阿魏酸酯、24-亚甲基环阿屯醇阿魏酸酯和β-谷甾醇阿魏酸酯，其含量分别为89.42、170.23和50.23毫克/100克，高分辨质谱法确认其分子量（m/z）分别为601.45、615.47和589.45。此外，通过氢核磁共振分析确认了[此处原文缺失具体香料名称]固定油中的γ-谷维素阿魏酸酯。固定油的酸化甲醇提取物表现出较高的自由基清除活性和抗氧化潜力。这些香料固定油具有出色的抗氧化活性，是对抗生活方式紊乱的新型潜在功能成分。
