开发了 198 个新型的基于 EST 的微卫星在桉树（桃金娘科）。

Development of 198 novel EST-derived microsatellites in Eucalyptus (Myrtaceae).

机构信息

State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Yiheyuan Hou, Beijing, People's Republic of China.

出版信息

Am J Bot. 2012 Apr;99(4):e134-48. doi: 10.3732/ajb.1100442. Epub 2012 Apr 2.

DOI:10.3732/ajb.1100442

PMID:22473983

Abstract

PREMISE OF THE STUDY

Expressed sequence tag (EST)-derived microsatellites were identified in Eucalyptus through screening the GenBank database. The loci were sequence-verified and explored for polymorphism among 20 genotypes.

METHODS AND RESULTS

In total, 198 novel microsatellites were developed from 8262 unigenes, with the identity of 73.6-100% to the original sequences and presence of the expected repeat motifs. One hundred and eighty-four markers proved to be polymorphic among 10 E. urophylla and 10 E. tereticornis genotypes, with the number of alleles per locus, observed heterozygosity, and polymorphic information content being 2-17 (mean: 7.11), 0-1.0 (mean: 0.4511), and 0.0940-0.9131 (mean: 0.6571), respectively.

CONCLUSIONS

These markers will be useful for germplasm characterization, genome mapping, and gene tagging for economic traits in the two species examined and may have potential for genetic applications in Eucalyptus.

摘要

研究前提

通过筛选 GenBank 数据库，在桉树中鉴定出表达序列标签（EST）衍生的微卫星。对这些基因座进行序列验证，并在 20 个基因型中探索其多态性。

方法和结果

总共从 8262 个基因中开发了 198 个新的微卫星，与原始序列的同一性为 73.6-100%，并存在预期的重复基序。在 10 个尾叶桉和 10 个赤桉基因型中，有 184 个标记被证明是多态的，每个基因座的等位基因数、观察杂合度和多态信息含量分别为 2-17（平均值：7.11）、0-1.0（平均值：0.4511）和 0.0940-0.9131（平均值：0.6571）。