Ainsworth J R, Lee W R
Department of Ophthalmology, University of Glasgow, Scotland.
Invest Ophthalmol Vis Sci. 1990 Apr;31(4):745-50.
An analysis of size, shape, vacuole and cell counts of Schlemm's canal was undertaken in 20 human eyes that had undergone conventional immersion fixation. A reduction in vacuole count with age was shown to be associated with a decreased canal cell count. The decrease in cell count with age was closely linked to an age-related reduction in the size of Schlemm's canal, notably a reduction in meridional width of the canal. An additional 18 human eyes underwent rapid perfusion fixation at 29.5 mm Hg to investigate the age-related response in the number of giant vacuoles present to this change in environment. A 4-fold increase in vacuole count compared to controls was demonstrable, but the numbers declined with age and the decline was closely correlated with a parallel reduction in cell numbers with age. It was possible to show that the reduction in cell and vacuole counts with age in the perfused eyes could be fully explained by the reduction in canal size: the numbers of vacuoles produced per cell and the vacuoles and cells per unit width of canal did not vary with age. It was also of interest that the pressure-related increase in vacuole numbers probably occurred within 15 min, which implies that in vivo the canal endothelium can respond quickly to changes in intraocular pressure.
对20只接受传统浸泡固定的人眼施累姆管的大小、形状、液泡及细胞计数进行了分析。结果显示，随着年龄增长，液泡计数减少与管细胞计数减少有关。细胞计数随年龄的减少与施累姆管大小的年龄相关性减小密切相关，尤其是管的子午线宽度减小。另外18只人眼在29.5毫米汞柱压力下进行快速灌注固定，以研究巨泡数量对这种环境变化的年龄相关反应。与对照组相比，液泡计数可证实增加了4倍，但数量随年龄下降，且这种下降与细胞数量随年龄的平行减少密切相关。可以证明，灌注眼中细胞和液泡计数随年龄的减少可完全由管大小的减小来解释：每个细胞产生的液泡数量以及每单位管宽度的液泡和细胞数量不随年龄变化。同样有趣的是，与压力相关的液泡数量增加可能在15分钟内发生，这意味着在体内，管内皮细胞可对眼压变化迅速做出反应。
