Lahlaoui H, Dahmen S, Moussa M B, Omrane B
laboratory of microbiology, Department of biology, Military Hospital of Tunis, Tunis, Tunisia.
Indian J Med Microbiol. 2011 Jul-Sep;29(3):258-61. doi: 10.4103/0255-0857.83909.
To study the resistance to third-generation cephalosporins in Providencia stuartii strain isolated from hospitalized patient in Tunisia and to identify the responsible genes
This strain was analysed by PCR and sequencing to identify the genes responsible for the β-lactamase resistance phenotypes. The transferability of the phenotypes was tested by conjugation to Escherichia coli J53. The isoelectric point was determinate by isoelectrofocalisation.
This resistance was carried by a 60 kb plasmid that encoded a β-lactamase with a pI of 5.4. This β-lactamase revealed identity with the blaTEM-1 gene encoding the TEM-1 β-lactamase, except for a replacement of the Val residue at position 84 by Ile, and the Ala residue at position 184 by Val. These two mutations were encountered in TEM-116 β-lactamase.
This study demonstrates the first description of TEM-116 in the P. stuartii species in the world and the first one in a Tunisian hospital.
研究从突尼斯住院患者中分离出的斯氏普罗威登斯菌菌株对第三代头孢菌素的耐药性，并鉴定相关基因。
通过聚合酶链反应（PCR）和测序分析该菌株，以鉴定负责β-内酰胺酶耐药表型的基因。通过与大肠杆菌J53进行接合试验来测试表型的可转移性。通过等电聚焦确定等电点。
这种耐药性由一个60 kb的质粒携带，该质粒编码一种等电点为5.4的β-内酰胺酶。除了第84位的缬氨酸残基被异亮氨酸取代，以及第184位的丙氨酸残基被缬氨酸取代外，这种β-内酰胺酶与编码TEM-1β-内酰胺酶的blaTEM-1基因具有同一性。这两个突变在TEM-116β-内酰胺酶中出现。
本研究首次在世界范围内报道了斯氏普罗威登斯菌中TEM-116，也是突尼斯一家医院中的首例。
