Mahrouki Sihem, Chihi Hela, Bourouis Amel, Ayari Khaoula, Ferjani Mustapha, Moussa Mohamed Ben, Belhadj Omrane
Laboratory of Biochemistry and Biotechnology, Department of Biology, Faculty of Sciences of Tunis, Campus Universitaire, 2092 El-Manar II, Tunis, Tunisia.
Laboratory of Biochemistry and Biotechnology, Department of Biology, Faculty of Sciences of Tunis, Campus Universitaire, 2092 El-Manar II, Tunis, Tunisia.
Diagn Microbiol Infect Dis. 2015 Jan;81(1):50-2. doi: 10.1016/j.diagmicrobio.2014.09.021. Epub 2014 Oct 2.
The aim of this study is to report the emergence of IncA/C conjugative plasmids harboring blaTEM-24, blaDHA-1, qnrA6, and aac(6')-Ib-cr genes among Providencia spp. isolates recovered in 2008 in Tunisia. The double-disk synergy test confirmed the phenotype extended-spectrum β-lactamase (ESBL) in 2 Providencia stuartii and 5 Providencia rettgeri. These ESBLs were coresistant to cefoxitin, chloramphenicol, ofloxacin, and sulfonamides but remained susceptible to imipenem. Three β-lactamases TEM-2, TEM-24, and DHA-1 were detected. blaTEM-24, blaDHA-1, aac(6')-Ib-cr, and qnrA6 genes were successfully transferred to Escherichia coli strain HB101, and they were found located on the conjugatifs IncA/C plasmids. Genetic relatedness showed similar and different patterns among P. stuartii and P. rettgeri strains, respectively.
本研究旨在报告2008年在突尼斯分离出的普罗威登斯菌属菌株中携带blaTEM-24、blaDHA-1、qnrA6和aac(6')-Ib-cr基因的IncA/C接合质粒的出现情况。双纸片协同试验证实2株斯氏普罗威登斯菌和5株雷氏普罗威登斯菌具有超广谱β-内酰胺酶（ESBL）表型。这些ESBL对头孢西丁、氯霉素、氧氟沙星和磺胺类药物耐药，但对亚胺培南仍敏感。检测到3种β-内酰胺酶TEM-2、TEM-24和DHA-1。blaTEM-24、blaDHA-1、aac(6')-Ib-cr和qnrA6基因成功转移至大肠杆菌HB101菌株，且发现它们位于接合性IncA/C质粒上。遗传相关性分析显示，斯氏普罗威登斯菌和雷氏普罗威登斯菌菌株分别呈现相似和不同的模式。
