Yamashita S, Maller J L
Department of Pharmacology, University of Colorado School of Medicine, Denver 80262.
J Cell Biol. 1990 May;110(5):1583-8. doi: 10.1083/jcb.110.5.1583.
Maturation-promoting factor (MPF) is a cell cycle control element able to cause cells to enter M-phase upon microinjection and will induce metaphase in nuclei incubated in cell extracts. Previous work has shown that MPF is composed of a complex between p34cdc 2 protein kinase and a B-type cyclin. In the present work gamma-S-ATP was found to cause activation of MPF activity in partially purified preparations, but this activation was lost upon chromatography on Matrex Green gel A. Readdition of other Matrex Green fractions to purified MPF restored the ability of gamma-S-ATP to activate MPF for nuclear breakdown as well as phosphorylation of histone H1. Use of the system described here will facilitate study of p34cdc 2 kinase activation and identification of elements involved in MPF regulation.
成熟促进因子（MPF）是一种细胞周期调控元件，微量注射后能使细胞进入M期，并能在细胞提取物中孵育的细胞核中诱导中期的出现。先前的研究表明，MPF由p34cdc 2蛋白激酶和B型细胞周期蛋白组成的复合物构成。在本研究中，发现γ-S-ATP能在部分纯化的制剂中激活MPF活性，但这种激活在Matrex Green凝胶A上进行层析后丧失。将其他Matrex Green组分重新添加到纯化的MPF中，可恢复γ-S-ATP激活MPF导致核破裂以及组蛋白H1磷酸化的能力。使用此处描述的系统将有助于研究p34cdc 2激酶的激活以及鉴定参与MPF调节的元件。
