Bachelerie F, Alcami J, Hazan U, Israël N, Goud B, Arenzana-Seisdedos F, Virelizier J L
Laboratoire d'Immunologie Virale, Institut Pasteur, Paris, France.
J Virol. 1990 Jun;64(6):3059-62. doi: 10.1128/JVI.64.6.3059-3062.1990.
Since human immunodeficiency virus (HIV) nef has been suggested to exert regulatory effects on HIV long terminal repeat (LTR) activity, we transiently transfected HIV LTR chloramphenicol acetyltransferase or luciferase expression vectors into a human astrocytoma clone (U-373nef) that constitutively expresses the HIV nef gene. In these cells, basal HIV LTR activity, as well as tumor necrosis factor-induced or tat-driven activity, was similar to that in control cells. Lack of any detectable effect of HIV nef on LTR activity was not the result of mutations in integrated nef DNA, as was shown by polymerase chain reaction. These data suggest that the role of nef in HIV genome transcription does not necessarily involve a direct influence on HIV LTR activity.
由于有人提出人类免疫缺陷病毒（HIV）的nef蛋白对HIV长末端重复序列（LTR）的活性具有调节作用，我们将HIV LTR氯霉素乙酰转移酶或荧光素酶表达载体瞬时转染到一个组成性表达HIV nef基因的人星形细胞瘤克隆（U - 373nef）中。在这些细胞中，基础HIV LTR活性以及肿瘤坏死因子诱导的或tat驱动的活性与对照细胞中的相似。聚合酶链反应结果表明，HIV nef对LTR活性缺乏任何可检测到的影响并非整合的nef DNA发生突变所致。这些数据表明，nef在HIV基因组转录中的作用不一定涉及对HIV LTR活性的直接影响。
