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1
Repressive effect of the nef cDNA of human immunodeficiency virus type 1 on the promoter activity of the viral long terminal repeat.
Jpn J Cancer Res. 1990 Nov;81(11):1124-31. doi: 10.1111/j.1349-7006.1990.tb02523.x.
2
Effects of the tat and nef gene products of human immunodeficiency virus type 1 (HIV-1) on transcription controlled by the HIV-1 long terminal repeat and on cell growth in macrophages.人类免疫缺陷病毒1型（HIV-1）的tat和nef基因产物对HIV-1长末端重复序列控制的转录以及巨噬细胞中细胞生长的影响。
J Virol. 1993 Dec;67(12):6956-64. doi: 10.1128/JVI.67.12.6956-6964.1993.
3
Human immunodeficiency virus type 1 (HIV-1) provirus expression and LTR transcription are repressed in NEF-expressing cell lines.在表达NEF的细胞系中，1型人类免疫缺陷病毒（HIV-1）前病毒表达和长末端重复序列（LTR）转录受到抑制。
Virology. 1991 Jun;182(2):522-33. doi: 10.1016/0042-6822(91)90593-z.
4
HIV-1 Nef alters the expression of betaII and epsilon isoforms of protein kinase C and the activation of the long terminal repeat promoter in human astrocytoma cells.HIV-1 Nef改变人星形细胞瘤细胞中蛋白激酶C的βII和ε亚型的表达以及长末端重复序列启动子的激活。
Glia. 1999 Aug;27(2):143-51.
5
Nef from primary isolates of human immunodeficiency virus type 1 suppresses surface CD4 expression in human and mouse T cells.来自人类免疫缺陷病毒1型原始分离株的Nef蛋白可抑制人和小鼠T细胞表面CD4的表达。
J Virol. 1993 Aug;67(8):4923-31. doi: 10.1128/JVI.67.8.4923-4931.1993.
6
Nef protein of human immunodeficiency virus type 1: evidence against its role as a transcriptional inhibitor.人类免疫缺陷病毒1型的Nef蛋白：反对其作为转录抑制剂作用的证据。
Proc Natl Acad Sci U S A. 1989 Dec;86(23):9549-53. doi: 10.1073/pnas.86.23.9549.
7
Human immunodeficiency virus-1 Nef protein interacts with Tat and enhances HIV-1 gene expression.人类免疫缺陷病毒1型Nef蛋白与Tat相互作用并增强HIV-1基因表达。
FEBS Lett. 2003 Jul 31;548(1-3):37-42. doi: 10.1016/s0014-5793(03)00725-7.
8
HIV-1 Nef protein inhibits the recruitment of AP-1 DNA-binding activity in human T-cells.HIV-1 Nef蛋白抑制人T细胞中AP-1 DNA结合活性的募集。
Virology. 1993 May;194(1):338-44. doi: 10.1006/viro.1993.1264.
9
Constitutive expression of human immunodeficiency virus (HIV) nef protein in human astrocytes does not influence basal or induced HIV long terminal repeat activity.人类免疫缺陷病毒（HIV）nef蛋白在人星形胶质细胞中的组成型表达不影响基础或诱导的HIV长末端重复序列活性。
J Virol. 1990 Jun;64(6):3059-62. doi: 10.1128/JVI.64.6.3059-3062.1990.
10
Effect of myristoylation on p27 nef subcellular distribution and suppression of HIV-LTR transcription.肉豆蔻酰化对p27 nef亚细胞分布及HIV-LTR转录抑制的影响。
Virology. 1992 Mar;187(1):46-55. doi: 10.1016/0042-6822(92)90293-x.

引用本文的文献

1
Pathogenesis of human immunodeficiency virus infection.人类免疫缺陷病毒感染的发病机制
Microbiol Rev. 1993 Mar;57(1):183-289. doi: 10.1128/mr.57.1.183-289.1993.
2
Expression kinetics and subcellular localization of HIV-1 regulatory proteins Nef, Tat and Rev in acutely and chronically infected lymphoid cell lines.HIV-1调节蛋白Nef、Tat和Rev在急性和慢性感染的淋巴细胞系中的表达动力学及亚细胞定位
Arch Virol. 1994;139(3-4):365-78. doi: 10.1007/BF01310798.
3
Identification of transcriptional suppressor proteins that bind to the negative regulatory element of the human immunodeficiency virus type 1.与人类免疫缺陷病毒1型负调控元件结合的转录抑制蛋白的鉴定
Nucleic Acids Res. 1991 Nov 25;19(22):6107-12. doi: 10.1093/nar/19.22.6107.

本文引用的文献

1
Expression and regulation of chicken actin genes introduced into mouse myogenic and nonmyogenic cells.导入小鼠成肌细胞和非成肌细胞的鸡肌动蛋白基因的表达与调控
Proc Natl Acad Sci U S A. 1984 May;81(10):2980-4. doi: 10.1073/pnas.81.10.2980.
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High-efficiency transformation of mammalian cells by plasmid DNA.质粒DNA对哺乳动物细胞的高效转化
Mol Cell Biol. 1987 Aug;7(8):2745-52. doi: 10.1128/mcb.7.8.2745-2752.1987.
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Cytoplasmic localization of the HTLV-III 3' orf protein in cultured T cells.人嗜T淋巴细胞病毒III型3' 开放阅读框蛋白在培养的T细胞中的细胞质定位。
Virology. 1986 Dec;155(2):593-9. doi: 10.1016/0042-6822(86)90219-9.
4
Effects of mutations within the 3' orf open reading frame region of human T-cell lymphotropic virus type III (HTLV-III/LAV) on replication and cytopathogenicity.人类嗜T淋巴细胞病毒III型（HTLV-III/LAV）3' 开放阅读框区域内突变对复制及细胞致病性的影响。
J Virol. 1986 Nov;60(2):754-60. doi: 10.1128/JVI.60.2.754-760.1986.
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Nef protein of HIV-1 is a transcriptional repressor of HIV-1 LTR.HIV-1的Nef蛋白是HIV-1长末端重复序列的转录抑制因子。
Science. 1988 Sep 16;241(4872):1481-5. doi: 10.1126/science.3262235.
6
HIV F/3' orf encodes a phosphorylated GTP-binding protein resembling an oncogene product.HIV F/3' 开放阅读框编码一种磷酸化的GTP结合蛋白，类似于一种癌基因产物。
Nature. 1987;330(6145):266-9. doi: 10.1038/330266a0.
7
Powerful and versatile enhancer-promoter unit for mammalian expression vectors.用于哺乳动物表达载体的强大且通用的增强子-启动子单元。
Gene. 1986;45(1):101-5. doi: 10.1016/0378-1119(86)90137-x.
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Demonstration of virus-specific transcriptional activator(s) in cells infected with HTLV-III by an in vitro cell-free system.
Cell. 1986 Oct 10;47(1):29-35. doi: 10.1016/0092-8674(86)90363-6.
9
Infectious mutants of HTLV-III with changes in the 3' region and markedly reduced cytopathic effects.3'区域发生变化且细胞病变效应显著降低的人嗜T淋巴细胞病毒III型感染性突变体。
Science. 1986 Aug 8;233(4764):655-9. doi: 10.1126/science.3014663.
10
Three novel genes of human T-lymphotropic virus type III: immune reactivity of their products with sera from acquired immune deficiency syndrome patients.人嗜T淋巴细胞病毒III型的三个新基因：其产物与获得性免疫缺陷综合征患者血清的免疫反应性
Proc Natl Acad Sci U S A. 1986 Apr;83(7):2209-13. doi: 10.1073/pnas.83.7.2209.

Repressive effect of the nef cDNA of human immunodeficiency virus type 1 on the promoter activity of the viral long terminal repeat.

作者信息

Mori S, Takada R, Shimotohno K, Okamoto T

机构信息

Virology Division, National Cancer Center Research Institute, Tokyo.

出版信息

Jpn J Cancer Res. 1990 Nov;81(11):1124-31. doi: 10.1111/j.1349-7006.1990.tb02523.x.

DOI:10.1111/j.1349-7006.1990.tb02523.x
PMID:2125037
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5917990/
Abstract

The nef gene product of human immunodeficiency virus type 1 (HIV-1) has been implicated as a negative factor for viral replication and is suspected to play an important role in the maintenance of viral latency. However, there seems to be evidence both for and against the negative effect of nef gene product. In the present report, we reevaluated the function of the nef gene by means of transient CAT assays with two human T cell lines. In most of the experiments, carefully controlled triplicate studies were carried out. We observed that not only the nef-expression plasmid, but also an effector plasmid containing the nef cDNA sequence in a reverse orientation, not expressing the Nef protein, showed a similar extent of repression of the HIV-1 promoter activity. We also examined the repressive effect of the nef cDNA with deletion mutants of HIV-1 long terminal repeat and heterologous promoters. The results led us to conclude that the apparent "repressor"-like action of the nef cDNA itself could be explained by competition for certain transcription factors required for HIV-1 gene expression by identical sequences also present in the nef cDNA.

摘要