Li Chen, Wei Yun-Shan, Wen Peng, Feng Kun, Zong Min-Hua, Wu Hong
School of Food Science and Engineering, South China University of Technology Guangzhou 510640 China
Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety Guangzhou 510640 China.
RSC Adv. 2018 Mar 8;8(18):9762-9769. doi: 10.1039/c8ra00385h. eCollection 2018 Mar 5.
A novel electrospun colon-specific delivery system for salmon calcitonin (SCT) was developed to improve its stability and bioavailability. Firstly, the pectin-coated SCT liposomes were prepared by film dispersion method and then a liposomes/sodium alginate/polyvinyl alcohol fiber mat was fabricated by electrospining. Scanning electron microscopy analysis indicated that the obtained nanofibers were uniform and smooth with an average diameter of about 350 nm. The release of SCT in different simulated digestive fluids was studied and corresponding release kinetics models were built. It was found that the fiber mat containing pectin-coated SCT liposomes had better stability and colon-specific properties compared with that containing uncoated SCT liposomes and the release of SCT in the colon followed the case II transport mechanism. In addition, there is no significant change in the bioactivity of released SCT measured by ELISA. This study shows that the electrospun colon-specific fiber mat is a potential delivery system for bioactive peptides.
为提高鲑鱼降钙素（SCT）的稳定性和生物利用度，开发了一种新型的电纺结肠特异性递送系统。首先，采用薄膜分散法制备了果胶包衣的SCT脂质体，然后通过静电纺丝制备了脂质体/海藻酸钠/聚乙烯醇纤维垫。扫描电子显微镜分析表明，所得纳米纤维均匀光滑，平均直径约为350nm。研究了SCT在不同模拟消化液中的释放情况，并建立了相应的释放动力学模型。结果发现，与含有未包衣SCT脂质体的纤维垫相比，含有果胶包衣SCT脂质体的纤维垫具有更好的稳定性和结肠特异性，且SCT在结肠中的释放遵循Ⅱ型转运机制。此外，通过酶联免疫吸附测定法测得的释放SCT的生物活性没有显著变化。本研究表明，电纺结肠特异性纤维垫是一种潜在的生物活性肽递送系统。
