State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, 2699 Qianjin Street, Changchun, 130012, China.
Lab Chip. 2011 Oct 7;11(19):3347-51. doi: 10.1039/c1lc20397e. Epub 2011 Aug 23.
We report here a facile approach for flexible integration of high efficiency surface enhanced Raman scattering (SERS) monitors in a continuous microfluidic channel. In our work, femtosecond laser direct writing was adopted for highly localizable and controllable fabrication of the SERS monitor through a multi-photon absorption (MPA) induced photoreduction of silver salt solution. The silver substrate could be shaped into designed patterns, and could be precisely located at the desired position of the microchannel bed, giving the feasibility for real-time detection during reactions. SEM and TEM images show that the silver substrates were composed of crystallized silver nanoplates with an average thickness of 50 nm. AFM results reveal that the substrates were about 600 nm in height and the surface was very rough. As representative tests for SERS detection, p-aminothiophenol (p-ATP) and flavin adenine dinucleotide (FAD) were chosen as probing molecules for microfluidic analysis at visible light (514.5 nm) excitation, exhibiting an enhancement factor of ~10(8). In addition, the combination of the SERS substrate with the microfluidic channel allows detection of inactive analytes through in situ microfluidic reactions.
我们在此报告一种灵活的方法，可将高效率表面增强拉曼散射（SERS）监测器集成到连续微流控通道中。在我们的工作中，采用飞秒激光直写技术，通过多光子吸收（MPA）诱导的银盐溶液光还原作用，实现 SERS 监测器的高定位和可控制造。银基底可以被塑造成设计的图案，并可以精确地位于微通道床的所需位置，为反应过程中的实时检测提供了可行性。SEM 和 TEM 图像显示，银基底由结晶银纳米板组成，平均厚度为 50nm。AFM 结果表明，基底的高度约为 600nm，表面非常粗糙。作为 SERS 检测的代表性测试，选择对氨基硫醇（p-ATP）和黄素腺嘌呤二核苷酸（FAD）作为可见光（514.5nm）激发下微流分析的探测分子，表现出约 10^8 的增强因子。此外，SERS 基底与微流道的结合允许通过原位微流反应检测非活性分析物。
