基于高效液相色谱的碳水化合物自动化合成与纯化

Department of Chemistry, Saint Louis University, 3501Laclede Ave, St. Louis, Missouri, 63103, USA.

Department of Chemistry, University of Milan, Via Golgi 19, Milan, 20133, Italy.

Chemistry. 2024 Aug 1;30(43):e202401214. doi: 10.1002/chem.202401214. Epub 2024 May 29.

Reported herein is a new HPLC-based automated synthesizer (HPLC-A) capable of a temperature-controlled synthesis and purification of carbohydrates. The developed platform allows to perform various protecting group manipulations as well as the synthesis of O- and N-glycosides. A fully automated synthesis and purification was showcased in application to different carbohydrate derivatives including glycosides, oligosaccharides, glycopeptides, glycolipids, and nucleosides.

本文报道了一种基于高效液相色谱的新型自动化合成仪（HPLC-A），该合成仪能够实现碳水化合物的温控合成和纯化。所开发的平台允许进行各种保护基操作以及 O-和 N-糖苷的合成。通过对包括糖苷、寡糖、糖肽、糖脂和核苷在内的不同碳水化合物衍生物的应用，展示了全自动合成和纯化的过程。