发夹结构增加了双链体的稳定性和碱基配对的选择性。
Intrastrand locks increase duplex stability and base pairing selectivity.
机构信息
Institute for Organic Chemistry, University of Stuttgart, Pfaffenwaldring 55, 70565 Stuttgart, Germany.
出版信息
Chem Commun (Camb). 2011 Oct 14;47(38):10824-6. doi: 10.1039/c1cc14008f. Epub 2011 Aug 24.
PMID:21863176
Abstract
Oligodeoxynucleotide probes with disulfide locks between neighboring nucleobases show increases in melting point for duplexes with RNA target strands of up to 7.6 °C. The weakly pairing TT dimers are replaced with locked 2'-deoxy-5-(thioalkynyl)uridine residues via automated synthesis.
摘要
寡核苷酸探针在相邻碱基之间具有二硫键锁，与 RNA 靶链形成的双链体的熔点升高高达 7.6°C。通过自动化合成，弱配对的 TT 二聚体被带有二硫键锁的 2'-脱氧-5-(硫代炔基)尿嘧啶残基取代。
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