不稳定非编码RNA的富集及其全基因组鉴定。

Enrichment of unstable non-coding RNAs and their genome-wide identification.

作者信息

Neil Helen, Jacquier Alain

机构信息

Unité de Génétique des Interactions Macromoléculaires, Institut Pasteur, CNRS, URA2171, Paris, France.

出版信息

Methods Mol Biol. 2011;759:87-106. doi: 10.1007/978-1-61779-173-4_6.

DOI:10.1007/978-1-61779-173-4_6

PMID:21863483

Abstract

Cryptic unstable transcripts (CUTs) have been recently described as a major class of non-coding RNAs. These transcripts are, however, extremely unstable in normal cells and their analyzes pose specific technical problems. In this chapter, after a brief introduction discussing general aspects associated with the analysis of non-coding RNAs, we provide details of methods to enrich, map, and quantify this unconventional class of transcripts.

摘要

隐秘不稳定转录本（CUTs）最近被描述为一类主要的非编码RNA。然而，这些转录本在正常细胞中极其不稳定，对它们的分析存在特定的技术问题。在本章中，在简要介绍与非编码RNA分析相关的一般方面之后，我们详细介绍了富集、定位和定量这类非常规转录本的方法。