[机会性筛查样本中高危型人乳头瘤病毒与宫颈病变的相关性研究]

[Study for association between high risk human papillomavirus and cervical lesions in the samples from opportunistic screening].

作者信息

Bian Li-hua, Li Ying, Wang Xiao-li, Wu Xiao-zhou, Song Lei, Liu Hong-tu

机构信息

Department of Obstetrics and Gynaecology, Chinese PLA General Hospital, Beijing 100853, China.

出版信息

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2011 Apr;25(2):89-91.

PMID:21863625

Abstract

OBJECTIVE

To investigate the association between HPV genotypes and cervical lesion in Hybrid Capture 2 (HC2) HPV test positive samples.

METHODS

602 cervical samples randomly obtained detected as HPV positive by the HC2 high-risk probe cocktail were determined by polymerase chain reaction (PCR)-reverse dot blot (RDB), among them 344 participated Thinprep Cytology test.

RESULTS

569 (94.5%) samples were successfully amplified. The most common HPV genotypes were HPV16 (31.6%), 52 (16.7%), 58 (15.1%), 56 (8.1%), 39 (7.9%); HPV16 or HPV16/18 infection was significantly related to ASCUS, LSIL and HSIL; 24 samples suggested single HPV infection with the genotypes not available for HC2.

CONCLUSION

The most common HPV genotypes in random screening were HPV16, 52 and HPV58; Our data demonstrated the pseudo-positivity of HC2 test was 4.0%; HPV16 was a index for progression of HSIL.

摘要

目的

探讨杂交捕获2代（HC2）HPV检测阳性样本中HPV基因型与宫颈病变之间的关联。

方法

通过聚合酶链反应（PCR）-反向斑点杂交（RDB）对随机采集的602份经HC2高危探针混合液检测为HPV阳性的宫颈样本进行检测，其中344份样本同时进行了薄层液基细胞学检测。

结果

569份（94.5%）样本成功扩增。最常见的HPV基因型为HPV16（31.6%）、52（16.7%）、58（15.1%）、56（8.1%）、39（7.9%）；HPV16或HPV16/18感染与非典型鳞状细胞（ASCUS）、低度鳞状上皮内病变（LSIL）和高度鳞状上皮内病变（HSIL）显著相关；24份样本提示单一HPV感染，其基因型在HC2检测中无法检出。